Standardization of the method for estimation of ethambutol in pharmaceutical preparations and biological fluid.
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2004-01-28
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Abstract
A simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an organic solvent, followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable eluants and estimation at a wavelength of 270 nm. The assay was linear from 25 to 400 microg/ml. The relative standard deviations of intra and inter day assays were lower than 5%. Ethambutol was recovered from human urine quantitatively and stable for a period of at least one week in urine stored at -20 degrees C.
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Gurumurthy P, Gayathri TN, Bhagavathy S, Venkatesan P. Standardization of the method for estimation of ethambutol in pharmaceutical preparations and biological fluid. Indian Journal of Experimental Biology. 2004 Jan; 42(1): 68-73