Standardization of the method for estimation of ethambutol in pharmaceutical preparations and biological fluid.

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dc.contributor.authorGurumurthy, Premaen_US
dc.contributor.authorGayathri, T Nen_US
dc.contributor.authorBhagavathy, Sen_US
dc.contributor.authorVenkatesan, Pen_US
dc.date.accessioned2009-05-28T14:28:16Z
dc.date.available2009-05-28T14:28:16Z
dc.date.issued2004-01-28en_US
dc.description.abstractA simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an organic solvent, followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable eluants and estimation at a wavelength of 270 nm. The assay was linear from 25 to 400 microg/ml. The relative standard deviations of intra and inter day assays were lower than 5%. Ethambutol was recovered from human urine quantitatively and stable for a period of at least one week in urine stored at -20 degrees C.en_US
dc.description.affiliationBiochemistry Department, Tuberculosis Research Centre (Indian Council of Medical Research), Chennai 600 031, India. icmrtrc@vsnl.comen_US
dc.identifier.citationGurumurthy P, Gayathri TN, Bhagavathy S, Venkatesan P. Standardization of the method for estimation of ethambutol in pharmaceutical preparations and biological fluid. Indian Journal of Experimental Biology. 2004 Jan; 42(1): 68-73en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/60750
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAntitubercular Agents --pharmacokineticsen_US
dc.subject.meshChemistry, Clinical --methodsen_US
dc.subject.meshChromatography --methodsen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshEthambutol --pharmacokineticsen_US
dc.subject.meshHIV Seropositivity --complicationsen_US
dc.subject.meshHumansen_US
dc.subject.meshIsoniazid --chemistryen_US
dc.subject.meshPharmaceutical Preparationsen_US
dc.subject.meshPyrazinamide --chemistryen_US
dc.subject.meshReference Standardsen_US
dc.subject.meshReproducibility of Resultsen_US
dc.subject.meshResins, Synthetic --chemistryen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshTime Factorsen_US
dc.titleStandardization of the method for estimation of ethambutol in pharmaceutical preparations and biological fluid.en_US
dc.typeJournal Articleen_US
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