Measurement of HDL-cholesterol in serum by a new enzymatic method.
dc.contributor.author | Tangvarasittichai, Surapon | |
dc.contributor.author | Taytiwat, Prawit | |
dc.date.accessioned | 2012-03-30T05:50:54Z | |
dc.date.available | 2012-03-30T05:50:54Z | |
dc.date.issued | 2001-07 | |
dc.description.abstract | High density lipoprotein (HDL) are responsible for the reverse transport of cholesterol from the peripheral cells to the liver. Here, cholesterol is metabolised. Monitoring of HDL-C in serum is of clinical importance since an inverse correlation exists between serum HDL-C concentrations and the risk of atheroselerotic disease. Elevated HDL-C concentrations are protective against coronary heart disease. In this study, an automated enzymatic method for the direct determination of HDL-C in serum was found to have good precision and accuracy. Reproducibility was determined daily using control precinorm? L in an internal protocol (n=20), within-run CV = 1.3% and between-day CV = 2.013%. The comparison of the HDL-C enzymatic assay with HDL-C precipitating agent was good with a correlation coefficient r = 0.965, Y = 1.981+ 0.959X, n =172. The normal range of HDL-C concentration in serum by this enzymatic method was 36.16 -86.08 mg./dl. Thus, a HDL-C level of over 35 mg/dl., shows the risk is not high and is protective against coronary heart disease. | en_US |
dc.identifier.citation | Tangvarasittichai Surapon, Taytiwat Prawit. Measurement of HDL-cholesterol in serum by a new enzymatic method. Siriraj Medical Journal, 2001 Jul; 53(7): 414-419. | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/137400 | |
dc.language.iso | en | en_US |
dc.source.uri | https://www.sirirajmedj.com/content_download.php?content_id=902 | en_US |
dc.subject | HDL-cholesterol | en_US |
dc.subject | New enzymatic method | en_US |
dc.title | Measurement of HDL-cholesterol in serum by a new enzymatic method. | en_US |
dc.type | Article | en_US |