Effect of different antipsychotics on cytokine production after immunologically stimulated PBMC culture.

dc.contributor.authorAl-Amin, Mamun
dc.date.accessioned2014-07-15T11:47:16Z
dc.date.available2014-07-15T11:47:16Z
dc.date.issued2013-01
dc.description.abstractAims: To investigate the effect of different antipsychotics on cytokine production in immunologically challenged Peripheral Blood Mononuclear Cell (PBMC) culture. Study Design: In vitro cell culture study to determine cytokine (IL-4, IL-10 and IFN-γ) level. Place and Duration of Study: Department of Pharmacy, North South University, Dhaka between January 2013 and April 2013. Methodology: Blood sample was collected from 22 healthy volunteers. Peripheral Blood Mononuclear Cells were separated and culture was prepared. The culture was stimulated with either LPS (lipopolysaccharide) or poly(I:C) (polyinosinic:polycytidylic acid). Stimulated PBMC culture was treated with typical antipsychotic (Haloperidol) and atypical antipsychotics (Clozapine, Quetiapine, Risperidone). Pro-inflammatory (IFN-γ) and antiinflammatory (IL-4, IL-10) cytokine levels were determined from the stimulated PBMC culture and stimulated plus antipsychotic treated PBMC culture. Results: Typical antipsychotic; Haloperidol and atypical antipsychotics; Clozapine, Quetiapine, Risperidone significantly (P = .05) enhance IL-10 production but not IL-4 in the LPS and poly(I:C) stimulated PBMC culture. IL-10 production was robust in LPS stimulated PBMC culture than the poly(I:C) stimulated culture. Typical and atypical both antipsychotics significantly (P = .05) reduce increased IFN-γ level in the LPS and poly(I:C) stimulated PBMC culture. Conclusion: Typical and atypical antipsychotics were successfully alters immune function by the suppression of pro-inflammatory cytokine (IFN-γ) levels and elevation of anti-inflammatory cytokine (IL-10).en_US
dc.identifier.citationAl-Amin Mamun. Effect of different antipsychotics on cytokine production after immunologically stimulated PBMC culture. International Neuropsychiatric Disease Journal. 2013 Jan-Jun; 1(1): 35-45.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/152647
dc.language.isoenen_US
dc.source.urihttps://www.sciencedomain.org/abstract.php?iid=237&id=29&aid=1544en_US
dc.subjectSchizophreniaen_US
dc.subjectcytokineen_US
dc.subjectantipsychoticen_US
dc.subjectlipopolysaccharideen_US
dc.titleEffect of different antipsychotics on cytokine production after immunologically stimulated PBMC culture.en_US
dc.typeArticleen_US
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