Effect of different antipsychotics on cytokine production after immunologically stimulated PBMC culture.
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Date
2013-01
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Abstract
Aims: To investigate the effect of different antipsychotics on cytokine production in
immunologically challenged Peripheral Blood Mononuclear Cell (PBMC) culture.
Study Design: In vitro cell culture study to determine cytokine (IL-4, IL-10 and IFN-γ)
level.
Place and Duration of Study: Department of Pharmacy, North South University, Dhaka
between January 2013 and April 2013.
Methodology: Blood sample was collected from 22 healthy volunteers. Peripheral Blood
Mononuclear Cells were separated and culture was prepared. The culture was stimulated
with either LPS (lipopolysaccharide) or poly(I:C) (polyinosinic:polycytidylic acid).
Stimulated PBMC culture was treated with typical antipsychotic (Haloperidol) and atypical
antipsychotics (Clozapine, Quetiapine, Risperidone). Pro-inflammatory (IFN-γ) and antiinflammatory
(IL-4, IL-10) cytokine levels were determined from the stimulated PBMC
culture and stimulated plus antipsychotic treated PBMC culture.
Results: Typical antipsychotic; Haloperidol and atypical antipsychotics; Clozapine,
Quetiapine, Risperidone significantly (P = .05) enhance IL-10 production but not IL-4 in
the LPS and poly(I:C) stimulated PBMC culture. IL-10 production was robust in LPS
stimulated PBMC culture than the poly(I:C) stimulated culture. Typical and atypical both
antipsychotics significantly (P = .05) reduce increased IFN-γ level in the LPS and poly(I:C) stimulated PBMC culture.
Conclusion: Typical and atypical antipsychotics were successfully alters immune
function by the suppression of pro-inflammatory cytokine (IFN-γ) levels and elevation of
anti-inflammatory cytokine (IL-10).
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Keywords
Schizophrenia, cytokine, antipsychotic, lipopolysaccharide
Citation
Al-Amin Mamun. Effect of different antipsychotics on cytokine production after immunologically stimulated PBMC culture. International Neuropsychiatric Disease Journal. 2013 Jan-Jun; 1(1): 35-45.