Inhibitory Activity of Ceiba' Honey against Escherichia Coli ATCC 33218
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Date
2022-06
Journal Title
Journal ISSN
Volume Title
Publisher
Applied Biology and Pharmaceutical Sciences
Abstract
Escherichia coli is a type of bacteria that has evolved yearly, such as resistance to various antibiotics. Honey consumption can improve the quality of individual health. It has the potential to inhibit the growth of Escherichia coli. This study aimed to characterize the Escherichia coli ATCC 33218, and to investigate the inhibitory activity of Ceiba' honey against this strain. We obtained Ceiba' honey from the honey farm of Ceiba petandra. The antibiotic sensitivity tests revealed that the strain used in this study is not ESBL strain. Even it is resistant to Ampicillin, Amoxycillin, Penicillin, and Oxacillin and is sensitive to Cefotaxime. This study runs inhibition zone, MIC, MBC, and time-kill growth experiments to evaluate the inhibitory activity of Ceiba' honey. It showed a strong inhibition zone. Its MIC and MBC were 30 and 40%, respectively. The time-kill growth experiments revealed its inhibition by lowering the lag phase, slowing the growth rate, and decreasing the biomass yield of strain. In conclusion, Escherrichia coli ATCC 33218 is not an ESBL strain. Furthermore, Ceiba' honey has inhibitory and killing power against Escherichia coli ATCC 33218.
Description
Keywords
Ceiba Pentandra, MIC, MBC, Madu Randu, Time-Kill Growth
Citation
Harly Michelle Angelica, Pasaribu Donna Mesina Rosadini, Timotius Kris Herawan. Inhibitory Activity of Ceiba' Honey against Escherichia Coli ATCC 33218. International Journal of Applied Biology and Pharmaceutical Technology. 2022 Jun; 13(2): 14-18