Urinary 8‑hydroxydeoxyguanosine as a marker of oxidative stress induced genetic toxicity in oral cancer patients.
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Date
2015-05
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Abstract
Context: Recently, non‑communicable diseases have snatched the lead from infectious diseases
in causing mortality. Of these, oral cancer accounts for a significant proportion of deaths. Every
year in India significant percentage of newly diagnosed malignancy is oral cancer attributed
to various reasons.
Aims: The aim of this study was to assess the extent of oxidative stress and its effect on
modification of DNA by urinary nucleoside 8-hydroxy-2’-deoxyguanosine (8-OHdG) levels in
oral cancer subjects. To see the relationship between the nucleoside 8‑OHdG and antioxidant
capacity ferric reducing ability plasma (FRAP) in oral cancer subjects.
Settings and Design: Case–control study included three groups with 60 volunteers, who
were divided into 30 controls, and equal number of clinically diagnosed oral cancer male
patients: (Subdivided into newly diagnosed [n = 15] and 1‑year treatment follow‑up oral cancer
subjects [n = 15]).
Materials and Methods: A random urine sample was used for analysis of 8‑OHdG concentration.
Serum triglycerides, lipid peroxidation, protein thiols, and FRAP assay were performed by
spectrophotometric technique.
Statistical Analysis Used: Student’s t‑test and one‑way analysis of variance were performed
for group comparison and Pearson’s correlation analysis were used. A P < 0.05 was considered
the optimum level of significance.
Results: The urinary 8‑OHdG and serum malondialdehyde levels were significantly elevated
in newly diagnosed oral cancer subjects in their 1‑year treatment compared to the control
group (P < 0.05). A significant correlation was observed between urinary 8‑OHdG and FRAP
in both groups of oral cancer subjects.
Conclusions: Urinary 8‑OHdG can be a useful diagnostic marker of oxidative DNA damage in
oral cancer subjects.
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Keywords
8‑hydroxy‑2’‑deoxyguanosine, ferric reducing ability plasma, marker, malondialdehyde, oral cancer
Citation
Murugaiyan Sathish Babu, Ramasamy Ramesh, Nakkeeran M, Rangdhol Vishwanath, Srinivasan A R, Niranjan G. Urinary 8‑hydroxydeoxyguanosine as a marker of oxidative stress induced genetic toxicity in oral cancer patients. Indian Journal of Dental Research. 2015 May-June; 26(3): 226-230.