Quality assessment of sputum for Acid Fast Bacilli(AFB) test in microbiology laboratories before and after implementation of Standard Operating Procedure (SOP): a comparative study.

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dc.contributor.authorKhadeza, K
dc.contributor.authorKamal, A H M
dc.contributor.authorNaima, M
dc.contributor.authorShamsuzzaman, S M
dc.date.accessioned2015-12-07T11:48:23Z
dc.date.available2015-12-07T11:48:23Z
dc.date.issued2012-01
dc.description.abstractAims: To evaluate the present status of microbiology laboratory by comparing the test results of investigator with that of laboratory staff for sputum for Acid Fast Bacilli (AFB) withZiehl-NeelsenStaining (Z-N Staining) at primary and secondary level andto assess the present status of a microbiology laboratory for sputum for Acid Fast Bacilli (AFB) withZiehl-NeelsenStaining (Z-N Staining) at tertiary level. Material and Methods: Type of study : Cross sectional, descriptive type of study. Place of study: Department of Microbiology of Dhaka Medical College,Narsingdi Sadar Hospital,Narsingdi,PolashUpzilla Health Complex ,Polash, Narsingdi andDOTS centers. Duration of study: From July, 2007 to June,2008 Method of sampling: Non probability, purposive and convenient sampling Sample Size: Sputum for AFB: 300 sputum samples were collected for detection of AFB by direct Z-N staining, Z-N staining after bleach centrifugation and Auramine phenol staining before implementing SOP. After following SOP 150 sputum samples were collected and tested in direct Z-N method. Results: In the present study, before SOP out of 100 sputum smear at each level, discrepancy was found in 3% cases at primary level, 2% cases at secondary level and 1% case at tertiary level.After following SOP out of 50 sputum smear at each level, discrepancy was reduced to 2% cases at primary level and no discrepancy was found at secondary and tertiary level. Conclusion: Each laboratory must have SOP for laboratory testing to set the minimum acceptable standard for every test in order to improve and maintain the quality of laboratory services.en_US
dc.identifier.citationKhadeza K, Kamal A H M., Naima M, Shamsuzzaman S M. Quality assessment of sputum for Acid Fast Bacilli(AFB) test in microbiology laboratories before and after implementation of Standard Operating Procedure (SOP): a comparative study. Bangladesh Journal of Medical Science. 2012; 11(1): 51-56.en_US
dc.identifier.issn2223-4721
dc.identifier.issn2076-0299
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/167394
dc.language.isoenen_US
dc.source.urihttps://www.banglajol.info/index.php/BJMS/article/view/9824en_US
dc.subjectQuality assessmenten_US
dc.subjectSputum for AFB testen_US
dc.subjectMicrobiology laboratoriesen_US
dc.subjectStandard Operating Procedure (SOP)en_US
dc.titleQuality assessment of sputum for Acid Fast Bacilli(AFB) test in microbiology laboratories before and after implementation of Standard Operating Procedure (SOP): a comparative study.en_US
dc.typeArticleen_US
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