Quality assessment of sputum for Acid Fast Bacilli(AFB) test in microbiology laboratories before and after implementation of Standard Operating Procedure (SOP): a comparative study.
Date
2012-01
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Abstract
Aims: To evaluate the present status of microbiology laboratory by comparing the test results of investigator
with that of laboratory staff for sputum for Acid Fast Bacilli (AFB) withZiehl-NeelsenStaining (Z-N
Staining) at primary and secondary level andto assess the present status of a microbiology laboratory for
sputum for Acid Fast Bacilli (AFB) withZiehl-NeelsenStaining (Z-N Staining) at tertiary level. Material
and Methods: Type of study : Cross sectional, descriptive type of study. Place of study: Department of
Microbiology of Dhaka Medical College,Narsingdi Sadar Hospital,Narsingdi,PolashUpzilla Health
Complex ,Polash, Narsingdi andDOTS centers. Duration of study: From July, 2007 to June,2008 Method
of sampling: Non probability, purposive and convenient sampling Sample Size: Sputum for AFB: 300 sputum
samples were collected for detection of AFB by direct Z-N staining, Z-N staining after bleach centrifugation
and Auramine phenol staining before implementing SOP. After following SOP 150 sputum samples
were collected and tested in direct Z-N method. Results: In the present study, before SOP out of 100 sputum
smear at each level, discrepancy was found in 3% cases at primary level, 2% cases at secondary level
and 1% case at tertiary level.After following SOP out of 50 sputum smear at each level, discrepancy was
reduced to 2% cases at primary level and no discrepancy was found at secondary and tertiary level.
Conclusion: Each laboratory must have SOP for laboratory testing to set the minimum acceptable standard
for every test in order to improve and maintain the quality of laboratory services.
Description
Keywords
Quality assessment, Sputum for AFB test, Microbiology laboratories, Standard Operating Procedure (SOP)
Citation
Khadeza K, Kamal A H M., Naima M, Shamsuzzaman S M. Quality assessment of sputum for Acid Fast Bacilli(AFB) test in microbiology laboratories before and after implementation of Standard Operating Procedure (SOP): a comparative study. Bangladesh Journal of Medical Science. 2012; 11(1): 51-56.