Screening and characterization of protease from bacillus sp.

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dc.contributor.authorSiddalingeshwara, K G
dc.contributor.authorUday, J
dc.contributor.authorHuchesh, C H
dc.contributor.authorPuttaraju, H P
dc.contributor.authorKarthic, J
dc.contributor.authorSudipta, K M
dc.contributor.authorPramod, T
dc.contributor.authorVishwanatha, T
dc.date.accessioned2015-06-19T10:26:13Z
dc.date.available2015-06-19T10:26:13Z
dc.date.issued2010-08
dc.description.abstractProtease production by alkalophilic Bacillus sp were isolated from different regions of Bangalore and used to screen for the protease production by using casein and skim milk agar plate assay. The agro wastes are also used to screen and produce protease. The agro wastes are rice bran, paddy straw and pigeon pea waste, among these substrate rice bran showed maximum synthesis of protease. At 48 hr it showed maximum 0.13 IU protease production in rice bran medium was used for partial purification studies. Fermented medium were used for salt precipitation, dialysis. The dayalyste were used for studies on effect of Ph, temperature, inhibitors and metal ions. The optimum pH 10, temperature 650C were optimum and the inhibitors and metal ion studies, metalions like Mn2+ and Ca2+ were found to be potent enhancers. The complete loss of enzyme activity was found in presence of PMSF.en_US
dc.identifier.citationSiddalingeshwara K G, Uday J, Huchesh C H, Puttaraju H P, Karthic J, Sudipta K M, Pramod T, Vishwanatha T. Screening and characterization of protease from bacillus sp. International Journal of Applied Biology and Pharmaceutical Technology. 2010 Aug-Oct; 1(2): 575-581.en_US
dc.identifier.issn0976-4550
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/158123
dc.language.isoenen_US
dc.source.urihttps://www.ijabpt.com/pdf/2447-Protease-IJAPART[1][2].pdfen_US
dc.subjectcasein-skim milk agaren_US
dc.subjectplate assayen_US
dc.subjectsolid state fermentationen_US
dc.subjectrice branen_US
dc.subjectal ions inhibitors and metal ionsen_US
dc.titleScreening and characterization of protease from bacillus sp.en_US
dc.typeArticleen_US
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