Screening and characterization of protease from bacillus sp.
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Date
2010-08
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Abstract
Protease production by alkalophilic Bacillus sp were isolated from different
regions of Bangalore and used to screen for the protease production by using casein and skim
milk agar plate assay. The agro wastes are also used to screen and produce protease. The agro
wastes are rice bran, paddy straw and pigeon pea waste, among these substrate rice bran showed
maximum synthesis of protease. At 48 hr it showed maximum 0.13 IU protease production in
rice bran medium was used for partial purification studies. Fermented medium were used for salt
precipitation, dialysis. The dayalyste were used for studies on effect of Ph, temperature,
inhibitors and metal ions. The optimum pH 10, temperature 650C were optimum and the
inhibitors and metal ion studies, metalions like Mn2+ and Ca2+ were found to be potent enhancers.
The complete loss of enzyme activity was found in presence of PMSF.
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Keywords
casein-skim milk agar, plate assay, solid state fermentation, rice bran, al ions inhibitors and metal ions
Citation
Siddalingeshwara K G, Uday J, Huchesh C H, Puttaraju H P, Karthic J, Sudipta K M, Pramod T, Vishwanatha T. Screening and characterization of protease from bacillus sp. International Journal of Applied Biology and Pharmaceutical Technology. 2010 Aug-Oct; 1(2): 575-581.