Depleted immunoglobulin heavy chain binding protein (BiP) expands the endoplasmic reticulum and the golgi apparatus in dengue virus-infected cells.
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Date
2012-01
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Abstract
Objective: To test whether depleted expression of immunoglobulin heavy chain binding protein (BiP), which is an endoplasmic
reticulum (ER) chaperone, in dengue virus (DENV)-infected cells, affected integrities of the ER and the Golgi apparatus of
the host cells.
Methods: Either siRNA against BiP- or control siRNA-transfected cells was infected with DENV and subjected to electron
microscopic evaluation.
Results: Depleted expression of BiP affected integrities of the ER and the Golgi apparatus in DENV-infected cells.
Conclusion: Integrities of the ER and the Golgi apparatus maintained by BiP in the host cells is necessary for DENV production.
Description
Keywords
Dengue virus, BiP, Endoplasmic reticulum, Golgi apparatus, RNA interference, Electron microscopy
Citation
Wongwiwat Wiyada, Niyomchan Apichaya, Yenchitsomaus Pa-thai, Sricharoenvej Sirinush, Limjindaporn Thawornchai. Depleted immunoglobulin heavy chain binding protein (BiP) expands the endoplasmic reticulum and the golgi apparatus in dengue virus-infected cells. Siriraj Medical Journal, 2012 Jan; 64(suppl): 91-92.