Molecular Characterization Of The First Record Of Entomopathogenic Fungi Beauveria Bassiana And Metarhizium Rileyi Isolates From Jharkhand, India
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Date
2024-01
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Publisher
Indian Society for Health and Advanced Research
Abstract
Two fungal isolates, resembling Beauveria bassiana and Metarhizium rileyi were collected and cultured from the ?elds of okra and groundnut at Ranchi, Jharkhand, India. This is the ?rst record of these fungi from Chotanagpur Plateau region. Their DNA was isolated and ampli?ed using the universal ITS1 and ITS4 primers. The ampli?ed product obtained was of the size of 500 bp for Beauveria bassiana and 550 bp for Metarhizium rileyi. Sequencing was performed using ITS1 primer. Comparing the respective sequences showed 97% resemblance to Beauveria bassiana and Metarhizium rileyi respectively. Multiple sequence alignment of ITS sequence of both the newly isolated strains along with its homologous sequences was conducted and a phylogenetic tree was built following the Neighbor-Joining method.
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Keywords
Beauveria bassiana, DNA Sequencing, Entomopathogenic fungi, Jharkhand, Metarhizium rileyi
Citation
Barla Cecilia K., Sharma Ajay K., Maurya S., Kumar Rahul . Molecular Characterization Of The First Record Of Entomopathogenic Fungi Beauveria Bassiana And Metarhizium Rileyi Isolates From Jharkhand, India . Indian Journal Of Applied Research. 2024 Jan; 14(1): 81-83