Hyphal Formation is Enhanced by 121°C Heat Inactivation of Serum Added to Culture Media
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Date
2020-05
Journal Title
Journal ISSN
Volume Title
Publisher
SCIENCEDOMAIN international
Abstract
Background: A novel method for inducing hyphae formation, was described.Methods:Mycelia of experimental and clinical strains from 100 patients with oral lichen planuswere cultured in RPMI 1640 medium with 56°C or 121°C inactivated calf sera. After these strains were cultured for 2 h to 7 days, the average hyphae formation rate was observed under light microscopy. Results: The 121°C inactivated calf serum showed better outcomes than 56°C inactivated calf serum in terms of time and amounts of hyphae products. Conclusions:For Candida albicanshyphae culture, the 121°C inactivated serum is superior to the 56°C inactivated serum
Description
Keywords
Serum inactivation, Candida albicans, hyphae, yeast, oral lichen planus
Citation
Hong He, Xueting Wang, Congcong Li, Xiaotong Deng, Yan Fan, Jianxin Han. Hyphal Formation is Enhanced by 121°C Heat Inactivation of Serum Added to Culture Media. Journal of Advances in Medicine and Medical Research. 2020 May; 32(6): 12-20