siRNAs targeting PB2 and NP genes potentially inhibit replication of Highly Pathogenic H5N1 Avian Influenza Virus.
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Date
2015-06
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Abstract
Highly Pathogenic Avian Influenza (HPAI) H5N1 virus is a threat to animal and public health worldwide. Till date,
the H5N1 virus has claimed 402 human lives, with a mortality rate of 58% and has caused the death or culling of
millions of poultry since 2003. In this study, we have designed three siRNAs (PB2-2235, PB2-479 and NP-865)
targeting PB2 and NP genes of avian influenza virus and evaluated their potential, measured by hemagglutination
(HA), plaque reduction and Real time RT-PCR assay, in inhibiting H5N1 virus (A/chicken/Navapur/7972/2006)
replication in MDCK cells. The siRNAs caused 8- to 16-fold reduction in virus HA titers at 24 h after challenged with
100TCID50 of virus. Among these siRNAs, PB2-2235 offered the highest inhibition of virus replication with 16-fold
reduction in virus HA titer, 80% reduction in viral plaque counts and 94% inhibition in expression of specific RNA at
24 h. The other two siRNAs had 68–73% and 87–88% reduction in viral plaque counts and RNA copy number,
respectively. The effect of siRNA on H5N1 virus replication continued till 48h (maximum observation period). These
findings suggest that PB2-2235 could efficiently inhibit HPAI H5N1 virus replication.
Description
Keywords
Avian influenza virus, NP, PB2, siRNA, subtype H5N1
Citation
Behera Padmanava, Nagarajan Shanmugasundaram, Murugkar Harshad V, Kalaiyarasu Semmannan, Prakash Anil, Gothalwal Ragini, Dubey Shiv Chandra, Kulkarni Diwakar D, Tosh Chakradhar. siRNAs targeting PB2 and NP genes potentially inhibit replication of Highly Pathogenic H5N1 Avian Influenza Virus. Journal of Biosciences. 2015 June; 40(2): 233-240.