Screening and Media Optimization for Enhancing L-asparaginase Production, an Anticancer Agent, from Different Filamentous Fungi in Solid State Fermentation.
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Date
2015
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Abstract
Aim: The aim of present study was to screen new potent fungal isolates and microorganisms
possessing extracellular L-asparaginase production capacity. In addition, optimization of cultural
and environmental conditions required for enzyme production will be carried out for the highest Lasparaginase
producer in solid state fermentation (SSF) technique using agro-industrial residues.
Study Design: Screening and physiological studies on the formation of L-asparaginase by
Trichoderma viride F2 in order to obtain the optimum cultural and environmental conditions
required for enzyme production.
Place and Duration of Study: Department of Microbial Chemistry, Genetic Engineering and
Biotechnology Division, National Research Centre (NRC), Cairo, Egypt, between July 2013 and
June 2015.
Methodology: Optimization of physical and nutritional parameters for enzyme production was
investigated. Various locally available agro-industrial residues have been screened individually or
as mixtures for L-asparaginase production. The combination of Rice husk (RH) with wheat bran (WB) (3:2) proved to be an efficient mixture for enzyme production as it gave the highest enzyme
activity (71.87±3.19 U/g-ds) when compared to individual RH (66.71±2.76 U/g-ds) or WB
(62.28±2.13 U/g-ds) substrates.
Results: Maximal L-asparaginase production (113.43±5.11 U/g-ds) by T. viride F2 was obtained
with moisture content of 75%, an inoculums size of 1 x 108 spores/ml and an initial medium pH of
5.0 when incubated at 28ºC for four days. Presence of Tween 20 enhanced enzyme production by
1.19 folds. Glucose (1.0%), Casein (1.5%) and MgCl2 (0.05%) were found to be the best carbon,
organic nitrogen and ion sources, respectively. Supplementation of the medium with NaNO3
(0.15%) as an inorganic nitrogen source further increased L-asparaginase production. Under these
optimized conditions, L-asparaginase production by T. viride F2 was maximum with a yield of
276.5±13.4 U/g-ds in SSF, which was more than 19-fold enhancement in enzyme activity as
compared to that obtained in the basal medium (SmF) (14.23±0.87 U/ml).
Conclusion: The results suggest that choosing a suitable substrate coupled with optimization of
different parameters can improves enzyme production markedly. Moreover, the production of Lasparaginase
from a process based on RH and WB as substrates in SSF is economically attractive
due to abundant substrates availability in agriculture-based countries with cheaper cost.
Description
Keywords
L-asparaginase, filamentous fungi, optimization, screening, solid state fermentation
Citation
Elshafei Ali Mohamed, El-Ghonemy Dina Helmy. Screening and Media Optimization for Enhancing L-asparaginase Production, an Anticancer Agent, from Different Filamentous Fungi in Solid State Fermentation. British Biotechnology Journal. 2015 9(3): 1-15.