Trans Sialidase Genes Allow Clustering of TcI Trypanosoma cruzi Mexican Isolates.
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Date
2014-12
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Abstract
Aims: Polymorphism of the trans sialidase (TS) family of genes is common in
Trypanosoma cruzi. Our goal was to cluster Mexican TcI DTU (Discrete Typing Unit) using
a set of primers specific for TS genes.
Methodology: The DNA of 12 Mexican T. cruzi stocks (TcI) and reference strain were
amplified using the CRP-1 primer, which anneals to the conserved 5' ends of CRP
(Complement Regulatory Protein), TS, and FL-160 genes, and the CRP-2 primer, which
anneals to conserved region within the GPI (Glycosil Phosphatidil Inositol) anchor
sequence. Amplicons were analysed using PCR-SSCP (Single Strand Conformation
Polymorphims) followed by construction of a nominal matrix data (presence/absence
bands) to calculated the Jaccard and Dice similarity coefficient, and clustering with
UPGMA.
Results: Mexican TcI stocks produced a common pattern of amplification products and
cluster in a separate group to CL-Brener strain (TcVI). The PCR-SSCP revealed that
within the TcI Mexican stocks there were a complex pattern, but T. cruzi from the Yucatan
peninsula clustered in special and separate group.
Conclusion: The CRP-1 and CRP-2 primers were helpful for the analysis of genetic traits
in T. cruzi DTU I and revealed the existence of special group in Yucatan Mexico.
Description
Keywords
Trypanosoma cruzi, discrete typing unit I, trans sialidase, PCR-SSCP
Citation
Monteón V M, López R, Ramos-Ligonio A, Acosta-Viana K. Trans Sialidase Genes Allow Clustering of TcI Trypanosoma cruzi Mexican Isolates. British Microbiology Research Journal. 2014 Dec; 4(12): 1299-1310.