Trans Sialidase Genes Allow Clustering of TcI Trypanosoma cruzi Mexican Isolates.

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dc.contributor.authorMonteón, V M
dc.contributor.authorLópez, R
dc.contributor.authorRamos-Ligonio, A
dc.contributor.authorAcosta-Viana, K
dc.date.accessioned2015-09-08T06:37:13Z
dc.date.available2015-09-08T06:37:13Z
dc.date.issued2014-12
dc.description.abstractAims: Polymorphism of the trans sialidase (TS) family of genes is common in Trypanosoma cruzi. Our goal was to cluster Mexican TcI DTU (Discrete Typing Unit) using a set of primers specific for TS genes. Methodology: The DNA of 12 Mexican T. cruzi stocks (TcI) and reference strain were amplified using the CRP-1 primer, which anneals to the conserved 5' ends of CRP (Complement Regulatory Protein), TS, and FL-160 genes, and the CRP-2 primer, which anneals to conserved region within the GPI (Glycosil Phosphatidil Inositol) anchor sequence. Amplicons were analysed using PCR-SSCP (Single Strand Conformation Polymorphims) followed by construction of a nominal matrix data (presence/absence bands) to calculated the Jaccard and Dice similarity coefficient, and clustering with UPGMA. Results: Mexican TcI stocks produced a common pattern of amplification products and cluster in a separate group to CL-Brener strain (TcVI). The PCR-SSCP revealed that within the TcI Mexican stocks there were a complex pattern, but T. cruzi from the Yucatan peninsula clustered in special and separate group. Conclusion: The CRP-1 and CRP-2 primers were helpful for the analysis of genetic traits in T. cruzi DTU I and revealed the existence of special group in Yucatan Mexico.en_US
dc.identifier.citationMonteón V M, López R, Ramos-Ligonio A, Acosta-Viana K. Trans Sialidase Genes Allow Clustering of TcI Trypanosoma cruzi Mexican Isolates. British Microbiology Research Journal. 2014 Dec; 4(12): 1299-1310.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/163267
dc.language.isoenen_US
dc.source.urihttps://sciencedomain.org/abstract/5412en_US
dc.subjectTrypanosoma cruzien_US
dc.subjectdiscrete typing unit Ien_US
dc.subjecttrans sialidaseen_US
dc.subjectPCR-SSCPen_US
dc.titleTrans Sialidase Genes Allow Clustering of TcI Trypanosoma cruzi Mexican Isolates.en_US
dc.typeArticleen_US
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