In-vitro Propagation of Aloe vera (L.) Burm. f.
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Date
2014-07
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Abstract
Aim: In vitro culture is used for commercial production and is achieved in aseptic
condition using different concentration and combination of Plant Growth Regulators
(PGR).
Material and Methods: In present work studied the effects of different plant growth
regulator singly or in combination in tissue culture of Aloe vera (L.) Burm. f. belongs to the
family Liliaceae or Asphodelaceae, used in ayurveda as well as pharmaceutical industry.
Murashige and Skoog (MS) media with different combinations and concentration of
growth promoters i.e. Auxin (Indole-3-butyric acid (IBA), α-Naphthalene acetic acid (NAA)
and Indole-3-acetic acid (IAA) and cytokinin (Benzyl Amino Purine (BAP) were used in
this study. Surface sterilization was standardised with HgCl2 with various concentration
and time.
Observation and Result: The development of callus type was observed in the MS media
supplemented with BAP for best regeneration, IBA for root formation and NAA, was found
the best media for root formation (0.5mg/l) were seen to grow onwards from the tenth day
of culture and 90% of root formation took place within a span of 3-4 weeks for maximum
callus induction. The maximum number of root & shoot produced is 4.8±0.53 with average
length of 3.5±0.35cm. Discussion: The present work deals with in vitro plant growth of Aloe vera through tissue
culture for propagation and ex situ conservation. Regenerated plants after acclimatization
were transferred to soil and they showed 85% survival. The culture response was
maximum in apical buds (100%) and minimum time required is 9.67 days in the same
media. The average length of shoots per culture was 4.0±0.16cm. In present study among
the three types of auxins, NAA was found to be the best for root induction.
Description
Keywords
Aloe vera, micropropagation, M S medium, in vitro, plant growth regulator
Citation
Gupta Sharmistha, Sahu Pankaj K, Sen Devki L, Pandey Priyanka. In-vitro Propagation of Aloe vera (L.) Burm. f. British Biotechnology Journal. 2014 Jul; 4(7): 806-816.