Metal binding to pyridoxal derivatives. An NMR study of the interaction of Eu(III) with pyridoxal phosphate and pyridoxamine phosphate.
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Date
1979-03
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Abstract
The solution conformations of pyridoxal-5'-phosphate and pyridoxamine-
5'-phosphate have been investigated using Eu(III) as a nuclear magnetic resonance
shift probe. Binding of Eu(III) to pyridoxal phosphate results in the formation
of two complexes, at the phosphate group and the o-hydroxy-aldehyde
moiety, which are in slow exchange on the nuclear magnetic resonance time-scale.
The lanthanide-induced pseudo contact shifts calculated using the McConnell-Robertson
equation (J. Chem. Soc. (1950), 22, 1561) are in good agreement with the
experimentally observed values for both pyridoxal phosphate and pyridoxamine
phosphate and lead to a family of closely related conformations.
Description
Keywords
Conformation, lanthanide, nuclear magnetic resonance, pyridoxal-5' phosphate, pyridoxamine-5'-phosphate
Citation
Rao B N Narasinga, Ramakrishnan C, Balaram P. Metal binding to pyridoxal derivatives. An NMR study of the interaction of Eu(III) with pyridoxal phosphate and pyridoxamine phosphate. Journal of Biosciences. 1979 Mar; 1(1): 35-47.