Interaction of amino acids with glycyl-glycine transport in the mammalian intestine.
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Date
1979-03
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Abstract
In order to investigate a possible interaction between free amino acids
and dipeptides during their mucosal uptake in man and monkey, perfusion studies
in vivo and uptake studies in vitro using labelled and non-labelled dipeptides and
amino acids have been carried out. In contrast to the observations of other workers,
inhibition of glycyl-glycine uptake was observed with free leucine and methioninc
but not with glycine, proline, hydroxyproline or alanine. Leucine and methionine
caused inhibition of cytosol glycyl-glycine hydrolase activity, while glycine had no
effect. The dipeptide uptake and dipeptide hydrolysis by cytosol enzyme was
competitively inhibited by leucine. Although brush border glycyl-glycine hydrolase
was also inhibited by leucine, the inhibition was noncompetitive. These data indicate
that a few free amino acids can interact with dipeptides during uptake. This
interaction might occur either at the transport step or at the stage of intracellular
dipeptide hydrolysis.
Description
Keywords
Intestine, peptide transport, peptidases, amino acid interaction
Citation
Ganapathy V, Hellier M D, Radhakrishnan A N. Interaction of amino acids with glycyl-glycine transport in the mammalian intestine. Journal of Biosciences. 1979 Mar; 1(1): 1-12.