Establishment of cDNA Library and EST Analysis from Leaves of Phyllanthus amarus.
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Date
2014-01
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Abstract
DNA sequencing of randomly chosen clones from a cDNA library allows thousands of
different transcripts to be identified. However, since the likelihood of observing a given
transcript is proportional to the expression level of that transcript in the tissue from which
the library is derived, often transcripts are represented by several EST sequences. An
expressed sequence tags (EST) analysis was undertaken to identify the genes present in
the leaves of Phyllanthus amarus, which is a small tropical, glabrous herb with several
health benefits. Phyllanthin and hypophyllanthin, major bioactive components, present in
highest amounts in the leaves, are of significant therapeutic importance like
hepatoprotective, antioxidant, antiviral, hypoglycemic, etc. Taken together, sequencing of
cDNA clones generated high-quality ESTs (Accession number: JK492908 to JK492964)
with high similarities with genes from Ricinus communis, Onchocerca volvulus, Eucalyptus
globules, Gossypium hirsutum, Nicotiana tabacum, Solanum spp. and many more. A
BLASTN analysis along with BLASTX analysis of all the unique sequences was performed
and was grouped according to the reported activities. Results represented here is the first
reference collection of ESTs from this commercially important medicinal herb. This study
indicated that the leaf transcriptome contains series of interesting sequences like
ALBINO3, ribulose-1, 5 bisphosphate carboxylase/ oxygenase (RUBISCO), chloroplast photosystem II chlorophyll A/B-binding protein, stress-responsive proteins like methionine
sulfoxide reductase type, etc.
Description
Keywords
cDNA library, expressed sequence tags (EST), Phyllanthus amarus
Citation
Chattopadhyay Sharmila, Bose Aparupa, Ghosh Mazumdar. Establishment of cDNA Library and EST Analysis from Leaves of Phyllanthus amarus. International Journal of Biochemistry Research & Review 2014 Jan-Feb ; 4 (1) : 1-15.