Establishing biological reference intervals for novel platelet parameters (immature platelet fraction, high immature platelet fraction, platelet distribution width, platelet large cell ratio, platelet-X, plateletcrit, and platelet distribution width) and their correlations among each other.
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Date
2014-04
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Abstract
Aims: This study aims to establish biological reference interval for novel platelet
parameters. Settings and Design: A total of 945 healthy individuals, age
ranges from 18 to 64 years (881 males and 64 females) coming for voluntary
blood donation from June to August 2012 (3 months) were enrolled after
exclusion of rejection criteria. Materials and Methods: The samples were
assayed by running in complete blood count + reticulocyte mode on the Sysmex
XE-2100 hematology analyzer and the reference interval for the population
was calculated using Clinical and Laboratory Standards Institute guidelines.
Statistical analysis used: Tests were performed using SPSS (Statistical Product
and Service Solutions , developed by IBM corporation), version 13. Student t
test and pearsons correlation analysis were also used. Results: The normal
range for various parameters was platelet count: 150-520 × 103/cu mm, immature
platelet fraction (IPF): 0.3-8.7%, platelet distribution width (PDW): 8.3-25.0 fL,
mean platelet volume (MPV): 8.6-15.5 fL, plateletcrit (PCT): 0.15-0.62%, high
immature platelet fraction (H-IPF): 0.1-2.7%, platelet large cell ratio (P-LCR):
11.9-66.9% and platelet-X (PLT-X) (ch): 11.0-22.0. Negative correlation was
observed between platelet count (r = −0.468 to r = −0.531; P < 0.001) and PCT
(r = −0.080 to r = −0.235; P < 0.05 to P < 0.001) with IPF, PDW, MPV, H-IPF,
P-LCR, and platelet-X. IPF/H-IPF showed a positive correlation among them and
also with PDW, MPV, P-LCR, platelet-X (r = +0.662 to r = +0.925; P < 0.001).
Conclusions: These novel platelet parameters offer newer avenues in research
and clinical use. Establishing biological reference interval for different platelet
parameters would help determine true high and low values and help guide
treatment decisions.
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Keywords
High immature platelet fraction, immature platelet fraction, platelet distribution width, platelet large cell ratio, reference interval
Citation
Sachdev Ritesh, Tiwari Aseem K, Goel Shalini, Raina Vimarsh, Sethi Monisha. Establishing biological reference intervals for novel platelet parameters (immature platelet fraction, high immature platelet fraction, platelet distribution width, platelet large cell ratio, platelet-X, plateletcrit, and platelet distribution width) and their correlations among each other. Indian Journal of Pathology & Microbiology. 2014 Apr-Jun 57 (2): 231-235.