Amelioration of inflammatory responses by Chlorogenic acid via suppression of pro-inflammatory mediators.
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Date
2011-06
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Abstract
The premise of the present investigation was to evaluate the detailed anti-inflammatory
properties of chlorogenic acid (CGA) and to delineate the possible mode of action. To explore
the anti-inflammatory profile, we evaluated the effect of CGA on TNF-alpha expression (in
vitro); carrageenan induced rat paw edema and carrageenan induce pleurisy (in vivo). In our
studies, CGA significantly inhibited the TNF-alpha expression, paw edema and antioxidant
enzymes in livers of rats in pre-treatment schedule but failed to exert any effect when
administered 1 h after carrageenan injection. CGA was also found to be safe, as confirmed by the
results of acute toxicity studies and MTT assay. CGA also caused reduction in total leucocytes
count most probably by inhibiting neutrophils, but could not alter mononuclear cells count in
carrageenan induced pleurisy. Inhibition of exudation was evidenced by the less exudate
formation in CGA treated animals, which may be due to decrease in vascular permeability which
was further confirmed in acetic acid induced vascular permeability model in mice where
significant decrease in vascular permeability was observed. CGA was highly effective in
reducing the arachidonic acid metabolites, nitric oxide and pro-inflammatory cytokines
production in a dose dependent manner and in some conditions effect observed was almost
comparable to ibuprofen. Result of the present investigation shows the anti-inflammatory effects
of CGS in different models.
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Keywords
Chlorogenic acid, pro-inflammatory cytokines, nitric oxide, PGE2, LTB4
Citation
Chauhan Prashant Singh, Satti Naresh Kumar, Sharma Vijay Kumar, Dutt Prabhu, Suri Krishan Avtar, Bani Sarang. Amelioration of inflammatory responses by Chlorogenic acid via suppression of pro-inflammatory mediators. Journal of Applied Pharmaceutical Science. 2011 Jun; 1(4): 67-75.