Journal of Medical Technology and Physical Therapy
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Item 20 Years of the Journal of Medical Technology and Physical Therapy(Khon Kaen University, 2009-12-25) Supan FucharoenNot availableItem Item 2007 Thai Journal Impact Factor: Top 10(Khon Kaen University, 2010-01-08) Supan FucharoenNot availableItem 2008 Thai Journal Impact Factor: Top 10(Khon Kaen University, 2009-12-24) Supan FucharoenAbstract not availableItem 2552 Thai Journal Impact Factor: Top 10(Khon Kaen University, 2010-12-21) Supan FucharoenAbstract not availableItem 5 Years international publication of the CMDL(Khon Kaen University, 2010-01-08) Supan Fucharoennot availableItem Abstracts, The 2007 Annual meeting of the Faculty of Associated Medical Sciences, 177 Khon Kaen University(Khon Kaen University, 2010-01-20) . .Abstract not availableItem The alteration of MHC class I expression in cervical cancer(Khon Kaen University, 2010-01-20) Patcharee Jearanaikoon; Nilna Kongmuangpuk; Umaporn Yodpratoom; Surasak Wanram; Chanvit Leelayuwat; Anucha Paupairoj; Temduang LimpaiboonIt was well established that HPV infection, especially type 16 and 18 is the major cause of cervical cancer. Immune evasion of infected cells or tumor cells is also known as one of contributing factors of tumor development caused by down-regulation of MHC class I expression. The aim of this study is to investigate the level and pattern of MHC class I expression in 182 cervical cancerous tissues and 57 normal cervix using immunoperoxidase staining technique. Of 182 cervical cancerous tissues, 50 cases were classified as pre-cancerous lesions whereas, 132 cases were invasive cervical carcinoma. No significant association in grading or intensity of MHC class I was shown between patient and control group. Interestingly, the distribution pattern of MHC class I was significantly shown as non-surface presenting molecules accumulating in cytoplasm of cancerous tissues compared to normal cervix tissues with p-value \< 0.001 χ² test .This result indicates that tumor cells have lost their immune surveillance by reducing their antigen presenting MHC class I molecules at cell surface. Moreover, this alteration can be used as an early marker for cancer prognosis in pre-cancerous group. Therefore, the mechanism of the impairment in antigen presenting of MHC class I molecules should be investigated in further study.Item Antifungal susceptibility of Candida species isolated from sterile sites of patients admitted in Srinagarind hospital(Khon Kaen University, 2009-12-25) Sukalya Srikulbutr; Keskaew Pienthaweechai; Supaporn Puapermpoonsiri; Churarat Pariyachartgul; Prajuab ChaimaneeThe minimum inhibitory concentrations (MIC) and susceptibility patterns of Candida species isolated from sterile sites of patients in Srinagarind Hospital were determined. The 46 isolates of Candida spp. compose of 24 isolates of Candida albicans, 9 isolates of Candida tropicalis, 5 isolates of Candida parapsilosis, 5 isolates of Candida glabrata; 2 isolates of Candida spp. and 1 isolate of C. krusei. All isolates were tested for MIC determination against 7 antifungal agents; amphotericin B, fluconazole, itraconazole, ketoconazole, flucytosine, voriconazole and caspofungin by Sensititre YeastOne colorimetric antifungal panel. All C. albicans isolates were susceptible to fluconazole, flucytosine and voriconazole whereas 96 % of them were susceptible to itraconazole. Similar results occurred when tested with other Candida spp. All of Candida spp. isolates were susceptible to voriconazole. Susceptibility to fluconazole, flucytosine and itraconazole were 77%, 91% and 55% respectively. MIC50 and MIC90 of C. albicans against amphotericin B were the same concentration at 0.5 mg/ml, fluconazole were 0.25 and 0.5 mg/ml, itraconazole were 0.032 and 0.064 mg/ml, ketoconazole were the same level at \< 0.008 mg/ml; flucytosine were 0.12 and 0.24 mg/ml; voriconazole were the same level at \< 0.008 mg/ml and caspofungin were the same concertration at 0.06 mg/ml. MIC50 and MIC90 of Candida spp. to amphotericin B were the same concentration at 0.5 mg/ml; fluconazole were 1 and 16 mg/ml respectively. The results showed that the first choice of antifungal agents was voriconazole because of 100 % susceptibility against both C. albicans and Candida spp. The second choices were flucytosine and fluconazole.Item Antimicrobial activity of partially purified bio-compounds of Brevibacillus laterosporus SA14 in saline with added amylase(Khon Kaen University, 2009-12-24) Darunee Chanwiriyagul; Benjamas Bunsongserm; Ratanaporn Kimaporn; Monthon LertcanawanichakulTo study antimicrobial activity of biocompounds produced from bacteria which may lead to development ofa model for antimicrobial substances production,Brevibacillus laterosporus SA14, isolated from air sample wasstudies. The partially purified biocompounds (PPBs) were prepared from 600 ml Luria Bertani culture broth of2 dayBrev. laterosporus SA 14 by means of 50 % ammonium sulfate precipitation. The PPbs could inhibitthe growth of Gram-positive bacteria (Staphylococcus aureus, Viridans group), Gram-negative bacteria (Escherichiacoli \& Pseudomonas aeruginosa) and yeast-like fungi (Candida albicans). The antimicrobial activity was decreasedwhen incubated with 1 mg/ml amylase for 5 hours at 37 oC. Moreover, the PPBs could show hemolytic activitywhich was disappeared at a titer higher than 1:8, but still had the antimicrobial activity when tested with agar welldiffusion. Interestingly, PPBs at a titer 1:128 could inhibit the growth of microorganisms in saliva when tested withmicrobroth dilution. It is therefore interesting to conduct further study on the application of this PPBs in productionof alcohol free mouthwash.Item Antimicrobial resistance co-transferred with extended-spectrum(Khon Kaen University, 2010-01-08) Aroonlug Lulitanond; Aroonwadee Chanawong; Pipat Sribenjalux; Nipada Klangjatturat; Kamolnate Pradudchonsee pdf of the full textItem Apolipoprotein E polymorphism and dyslipidemia in Thais(Khon Kaen University, 2009-12-24) Nisa Decharatkulchai; Chatri Settasatian; Nongnuch Settasatian; Nantarat Komanasin; Naruemon Leelayuwat; Suchart Sirijaichingkul; Yaovalak Teerajetgul; Duangrudee ChangtrakunApolipoprotein E (apoE) is a constituent on lipoprotein surface and plays an important role in lipid metabolism. There are three common isoforms of human apoE, designed apoE2, apoE3 and apoE4 that are coded by three polymorphic alleles of the APOE genes, e2, e3 and e4. Polymorphism of APOE influences the blood lipid concentration and may contribute to susceptibility to dyslipidemia. The present study was therefore to investigate the role of APOE polymorphism on blood lipid levels in Thai individuals. A total of 121 normolipidemic and 125 dyslipidemic subjects were recruited. DNA was isolated from peripheral blood leukocytes and APOE genotypes were determined by PCR-RFLP. Allele frequencies of e2, e3 and e4 were 9.3%, 77.8% and 12.8%, respectively. The respective prevalence of APOE genotypes for e2/e2, e3/e3, e4/e4, e2/e3, e2/e4 and e3/e4 were 1.2%, 62.2%, 0.8%, 11.8%, 4.5% and 19.5%. The frequencies of APOE allele and genotype of both groups as well as the relation of APOE genotypes to the risk of dyslipidemia were not significantly different. The elevation of some lipid parameters observed in male subjects and the higher level of lipid profile in dyslipidemic group may be due to other factors and gene polymorphisms.Item Assessment of quality control producy for thalassemia screening using MCV and MCH approaches(Khon Kaen University, 2009-12-24) Duangrudee Changtrakun; Kanokwan Sanchaisuriya; Goonnapa Fucharoen; Yossombat Changtrakun; Supan FucharoenA combined MCV/DCIP or MCH/DCIP is one of screening approach for thalassemia alternative to the OF/DCIP. Based on this approach, the MCV and MCH values obtained from electronic blood cell counters are of important that should be accurate and comparable. To evaluate the accuracy of thalassemia screening using the MCV and MCH measured from different instruments and settings, two quality control (QC) products, the RBC_A (MCV \> 80 fl and MCH \> 27 pg) and RBC_B (MCV \< 80 fl and MCH \< 27 pg), were prepared and sent to 13 participated laboratories. Rbc parameters of each QC product were measured routinely in each laboratory. By comparing to the initial values obtained in our laboratory, it was found that all Rbc parameters of the two QC products were accurately measured with the coefficient of variation (% CV) of 2.09 - 9.76 % for RBC_A and 1.11 - 11.04 % for RBC_B. Regarding to the instruments, the % CV of most Rbc parameters were less than 2 % for both RBC_A and RBC_B. To evaluate the accuracy of the MCV and MCH in application to thalassemia screening, the MCV cutoff value of 80 fl and MCH cutoff value of 27 pg were applied. The RBC_A was treated as negative sample and the RBC_B was treated as positive sample. By using the MCV approach, false negative results were obtained form 2 laboratories. In contrast, neither false negative nor false positive result was found with the MCH approach, suggesting that MCH might be more appropriate for thalassemia screening than MCV. The results indicate that these QC products might be used as an internal QC to evaluate the accuracy of Rbc parameters and as an external QC to evaluate the performance of thalassemia screening using the MCV and MCH approaches of each laboratory setting.Item Assessment of risk of falling in community-dwelling elderly by using the(Khon Kaen University, 2010-07-15) Nomjit Nualnetr; Juntimaporn Sriruang; Rattana Boonmat; Arada Chaiyamoon; Sirima BoonyaratFalls are a cause of health problem and lead to disability or death in the elderly. Prevention of falls, especially in the high risk group, is thus important with the determination of overall risk factors. The Thai Fall Risk Assessment Tool (Thai FRAT) is a test for evaluating the risk of falls by considering both intrinsic and extrinsic risk factors. Meanwhile the Berg Balance Scale (BBS) emphasizes only the intrinsic factors. This study aimed to examine the agreement of the Thai FRAT and the BBS on identifying the risk of falls in 47 community-dwelling elderly (18 men and 29 women, mean age 76.3 ± 4.2 years) of Samliam Community, Khon Kaen Province. The agreement of the Thai FRAT and the BBS was analyzed by Kappa statistic. The result showed that the agreement of the Thai FRAT and the BBS on identifying the elderly at risk for falling was fair (K = 0.33, P = 0.009). This finding may indicate that physical therapists should apply the Thai FRAT and the BBS for assessing risk of falls of the elderly in community to obtain both intrinsic and extrinsic risk factors.Item The association of Gγ XmnI polymorphism with hemoglobin F levels in homozygous hemoglobin E(Khon Kaen University, 2010-01-20) Naruwat Pakdee; Komgrid Sukmaitree; Goonnapa Fucharoen; Supan Fucharoen; Kanokwan Sanchaisuriya; Nattaya Sae-ungThe Gγ -Xmn I polymorphism (-158 C → T) in 131 subjects with homozygous Hb E were investigated. The Xmn I (+/+), (+/-) and (-/-) patterns were identified in 77 (58.8 %), 45 (34.3 %) and 9 (6.9 %) cases, respectively. This result indicates that majority of βE globin genes are linked to the Xmn I + allele. Among 61 cases of Hb E homozygote (Hb \> 9 g/dl) who had (bE/bE, aa/aa) genotype, 37 (59.7 %), 20 (32.3 %) and 5 (8.0 %) cases had the Xmn I (+ / +), (+ / -) and (- / -) patterns, respectively. The median levels of Hb E and other Rbc parameters in these groups were not difference. However, the median levels of Hb F as determined by Hb-HPLC analysis, the MCHF (ng) and the Hb F concentration (mg/dl) were significantly higher in the Xmn I (+ / +) group (3.6 %, 731.0 ng and 374.0 mg/dl) as compared to those with the Xmn I (+ / -) (2.4 %, 456.9 ng and 264.4 mg/dl) and the Xmn I (- / -) (2.5 %, 518.0 ng and 245.0 mg/dl) at p-value \< 0.01 using Kruskal Wallis One-way Analysis of Variance and Mann-Whitney U-Test. No difference was found between the Xmn I (+ / -) and the Xmn I (- / -) groups. Although the Gγ -Xmn I (+ / +) polymorphism is associated with high Hb F production in Hb E homozygote, it is apparent that other factors requiring further investigation might also be involved.Item Bacteriophage therapy(Khon Kaen University, 2009-12-24) Parichart PhumkhachornBacteriophages or phages are bacterial viruses or viruses that infect bacteria. Bacteriophages can have either a lytic or a lysogenic life cycle. The lytic phages are the most suitable candidates for phage therapy because they can kill the bacterial host at the end of replication cycle. Bacteriophages are highly specific to their hosts. When a bacteriophage meets a suitable host bacterium, its specific surface molecule binds to the particular molecule on a host and then injects its genetic material into the cell. In the host cell, bacteriophage instructs the machinery of the host to make more bacteriophages. Fully viable progeny bacteriophages burst out and kill the bacteria. The released bacteriophages attack new bacteria. This process continues until all the bacteria are eliminated from the system. Bacteriophage therapy was used as therapeutic agents in 1920. It was tried extensively and many successes were reported for a variety of infectious diseases including dysentery, staphylococcal skin infection, lung and pleural infection, and gastrointestinal tract infection. Lytic phages are similar to antibiotics in that they have remarkable bactericidal activity. However, therapeutic phages have some unique advantages over antibiotics. Nowadays, drug-resistant bacteria emerge rapidly so phage therapy is more attractive to be an alternative strategy to treat those bacteria. However, there are some problems with phage treatment works that must be addressed before it can be widely approved for therapeutic use.Item Balance and obstacle crossing ability in independent ambulatory spinal cord injury patients(Khon Kaen University, 2009-12-24) Sugalya Amatachaya; Kitti Sombanda; Kalyarat Rungratanachiwin; Sukanya Jaikla; Wantana Siritaratiwat; Worawan KamruechaPatients with spinal cord injury (SCI) are frequently having impairment of movement control particularly a complex task such as walking and balance control. This study investigated ability of 18 independent ambulatory SCI patients to control their balance by using Berg Balance Scale (BBS), Timed Up and Go Test (TUGT) and to walk over small obstacles (wide or high obstacles at the size 1, 4 and 8 cm., 6 conditions totally), which are the sizes that commonly found at home and in the community. Each obstacle was placed at the middle of the 10-meter walkway. Results demonstrated that most of independent ambulatory subjects still had problem in balance control that resulting in exposing to a high risk of fall (BBS = 32.83 + 13.66 scores and TUGT = 45.57 + 18.83 seconds). In addition, 39 % of subjects were unable to successfully walk over obstacle(s). There were clinical significance of balance control (BBS) between subjects who were able and unable to successfully walk over an obstacle (\> 6 scores). The results may indicate that the development of balance control and obstacle crossing in patients with SCI who are able to walk independently is important to decrease risk of accident after discharge.Item Balance, fall and quality of life in active and inactive elderly(Khon Kaen University, 2010-12-21) Thiwaporn Thaweewannakij; Sugalya Amatachaya; Punnee Peungsuwan; Lugkana MatoThis study aimed to assess the ability of balance control, fall, and quality of life in active and inactive elderly. In active elderly, the study also compared the information in elderly who regularly participated in exercise programs (exercise subjects) and routinely conducted physical activities (lifestyle active subjects). One hundred-and-fifty subjects from several communities in Khon Kaen province, Thailand, aged between 65 to 80 years with a BMI of 20-30 kg/m2 Participated in the study. They were subdivided into 3 groups (50 subjects/group). Each of them was tested on balance ability by using the timed up and go test (TUGT) and berg balance scale (BBS). They were also interviewed regarding their fall history during the past 6 months by using a questionnaire and quality of life by using a self-administered WHOQOL-BREF-THAI questionnaire. There were no significant differences on age, BMI and genders of subjects among the groups. Both exercise and lifestyle active subjects reported a higher rate perceived exertion score (Borg score) of their daily physical or exercise activity than that of inactive subjects (P \< 0.001). Lifestyle active subjects also had the longest duration of their routine physical activities. The results of balance performance showed that exercise subjects achieved the best balance control, followed by lifestyle active and inactive subjects respectively. Inactive subjects required the time to complete TUGT longer than 12 seconds (normative cut-off point for elderly between 65 and 85 years of age). Furthermore, inactive and lifestyle active subjects required the time to complete TUGT longer than exercise subject which was clinically significant from that of the exercise subjects. In addition, the number of inactive subjects who experienced falls during the past 6 months was about 2 times greater than that of active subjects. However, there were no significant differences in quality of life of subjects among the groups (P \> 0.05). Findings of this study confirm that the regular exercise is the best for balance control and fall prevention. However, participation in regular physical activity also benefits for those abilities. Thus the program for balance improvement and fall prevention in elderly should emphasize on regular exercise or physical activity.Item Blood stock in 2010 for Srinagarind hospital and Queensirikit Heart Center of the Northeast(Khon Kaen University, 2010-07-15) Amornrat Romphruk; Chintana PaupairojIt is the responsibility of all blood transfusion laboratories to ensure that donated blood is used efficiently and effectively and to minimize waste while maintaining sufficient stock to deal with unexpected life-threatening emergencies. This study was conducted to determine the adequate stock level needed to operate the Blood Transfusion Center of the Faculty of Medicine, Khon Kaen University. We accessed the total number of transfusion units and cross-matching of blood components from our data base for 2009. The average daily weekly and monthly use was calculated. A stock of blood components (viz., red cells and plasma) is kept for 7 and 3 days, respectively. Stocks of platelets are kept for 3 days and cryoprecipitate for 4 weeks. The stock levels included blood for emergencies (10 %) and to cover the expected growth in use over the year before (based on historical growth rates/usage). The results of our study showed an increased utilization in 2009 of: red cells 3.1 % (28,202 units); plasma 14.1 % (18,268 units), platelet concentrates 20.3 % (22,754 units) and cryoprecipitates 28.0 % (11,178 units). The ideal blood inventory of red cell products in 2010 should be 766 units; 158, 266, 288 and 54 units of blood group A, B, O and AB, respectively. The respective blood inventory for plasma, platelets and cryoprecipitates should be 548, 238, 1297 units. We also found that the red cell products would cover 3 days in the event of a shortage in the blood supply. The ideal blood inventory for our Blood Transfusion Center will be based upon this evidence. However, the inventory needs to be frequently checked for quality assurance and up-to-date stock counts. When the inventory level gets low, planning for restocking the blood supply should be done immediately as per policy. A statistical report on inventory levels throughout the year should be done each year for planning and evaluation purposes.Item Capability of electronic-filter air purifier on airborne microorganism removal(Khon Kaen University, 2009-12-24) Yuparat Limmongkol; Pipat Sribenjalux; Pipat Sribenjalux; Paradee Chuaybamroong; Paradee ChuaybamroongThe performance of the electronic-filter air purifier that uses the principle of electrostatic field on airborne fungi(Aspergillus niger and Penicillium citrinum) and bacteria (Staphylococcus epidermidis and Bacillus subtilis)removals was tested in this study. The experiment was conducted in the 8-m3 chamber by injecting each type ofmicroorganism and sampling the bioaerosol at two height levels, 0.5 and 1.5 m. Since the air purifier was locatedat the floor with the height of 0.67 m, the microbe concentrations at these two height levels were observed for anydifference in its performance. However, the main objective was to compare the microbe concentrations whenturning on the air purifier and turning off for duration of 4 hours. The results were that the air purifier could removethe microbes from the initial concentrations of 34,000 - 80,000 cfu/m3 to comply with the recommended concentrationof 500 cfu/m3 within 30-40 min except for B. subtilis that needed longer time. The height level did not affectthe performance of the air purifier in this study at the level of confidence of 99 %.