Browsing by Author "Kurup, P A"
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Item Accumulation of glycosaminoglycans associated with hypomagnesaemia in endomyocardial fibrosis in Kerala: possible involvement of dietary factors.(1997-01-01) Kumari, K T; Ravikumar, A; Kurup, P ANutritional factors, mainly low protein intakes have been implicated in the pathogenesis of endomyocardial fibrosis (EMF), the incidence of which is high in Kerala. As there is only marginal protein deficiency in the Kerala population, this may not be a causative factor. Studies have revealed low levels of magnesium (Mg) in the serum of these patients and high concentration of glycosaminoglycans. Accumulation of glycosaminoglycan-associated Mg deficiency is observed in the serum of EMF patients. The heart tissue from autopsy samples of EMF patients also showed accumulation of glycosaminoglycans.Item Activity of glycohydrolases of tissues in alloxan diabetic rabbits.(1978-08-01) Chempakam, B; Kurup, P AItem Adrenocortical suppression & lysosomal stability.(1978-06-01) Philip, B; Kurup, P AItem Alpha-amylase of the hepatopancreas of the scorpion, Heterometrus scaber.(1969-10-01) Vijayalekshmi, N R; Kurup, P AItem Antiatherogenic effect of a low lysine: arginine ratio of protein involves alteration in the aortic glycosamihoglycans and glycoproteins.(1990-12) Rajamohan, T; Kurup, P AThe effect of alteration of lysine: arginine ratio of the protein on the aortic glycosaminoglycans and glycoproteins was studied in rats fed cholesterol free and atherogenic diet. The concentration of total glycosaminoglycans and of individual fractions was significantly lower in the aorta in the case of diet with lysine: arginine ratio of 1·0, than the diet with a ratio of 2·0. Rats fed globulin fraction isolated from sesame seeds, which has a lysine: arginine ratio of 0·67 also showed significantly lower concentration of total and individual glycosaminoglycan fractions in the aorta than those fed casein (lysine:arginine ratio 2·0). Concentration of total hexose and fucose in the glycoproteins was also lower in the aorta in the case of lysine: arginine ratio 1·0. These results in the light of previous reports of increase in the aortic glycosaminoglycans in the early stages of atherosclerosis and increase in the total hexose and fucose in the glycoproteins in the atherosclerotic aorta indicate that the antiatherogenic effect of a low lysine: arginine ratio in the protein involves alteration in the aortic glycosaminoglycans and glycoproteins.Item Antibacterial principle of the root bark of Calophyllum inophyllum: isolation and antibacterial activity.(1970-01-01) Potti, G R; Kurup, P AItem Aortic/glycosaminoglycans alterations in antiatherogenic action of dietary fiber from unripe banana (Musa paradisiaca).(1991-04-01) Usha, V; Vijayammal, P L; Kurup, P ADietary fiber isolated as neutral detergent residue from unripe banana altered the concentration of aortic glycosaminoglycans in rats fed cholesterol free and cholesterol diet. Concentration of hyaluronic acid (9.9%), heparan sulphate (53.4%), chondroitin 4-sulphate (32.6%), chondroitin 6-sulphate (17.9%), dermatan sulphate (18.8%) and heparin (10.1%) increased in the aorta in rats fed cholesterol free diet. In rats fed cholesterol diet, concentration of heparan sulphate (23.3%), chondroitin 4-sulphate (9.8%) and heparin (42.4%) increased while hyaluronic acid showed a decrease (29.7%). The activity of beta-glucuronidase (9.5%) and beta-hexosaminidase (19.7%) decreased in the aorta in rats fed cholesterol free diet and given dietary fiber, while only beta-hexosaminidase (19.3%) decreased in rats fed cholesterol diet.Item Biotin deficiency causes alterations in glycosaminoglycans and glycoproteins in rat aorta.(1993-02-01) Abraham, A; Kurup, P ABiotin deficiency produced by feeding raw egg white caused decrease in the concentration of many glycosaminoglycans (GAG) in the aorta in rats fed standard laboratory diet and atherogenic diet. This decrease has been observed to be due to the increased activity of enzymes involved in the degradation of GAG and glycohydrolases which cleave the carbohydrate components of glycoproteins.Item Carbohydrate metabolism in the scorpion, Heterometrus scaber (Thor.): some enzymes of glycolysis, pentose phosphate pathway and citric acid cycle.(1976-03-01) Vijayalakshmi, N R; Kurup, P AItem Changes in carbohydrate components of glycoprotein in tissues other than vascular tissue in atheromatous rats.(1977-09-01) Satakopan, V N; Kurup, P AItem Changes in carbohydrate components of glycoproteins in tissues with alteration in the pyridoxine intake in rats fed normal & atherogenic diet.(1977-07-01) Vijayammal, P L; Kurup, P AItem Changes in carbohydrate components of glycoproteins of aorta, cartilage & serum in rats fed atherogenic diet.(1978-03-01) Satakopan, V N; Kurup, P AItem Changes in glycoproteins in isoproterenol-induced myocardial infarction in rats.(1982-02-01) Mathew, S; Menon, P V; Kurup, P AItem Changes in myocardial & aortic lipids, lipolytic activity & fecal excretion of sterols & bile acids in isoproterenol-induced myocardial infarction in rats.(1981-04-01) Mathew, S; Menon, P V; Kurup, P AItem Changes in soluble and insoluble glycoproteins in isoproterenol-induced myocardial infarction in rats.(1984-12-01) Mathew, S; Kurup, P AItem Changes in soluble glycoproteins in different tissues of alloxan diabetic rabbits.(1980-12-01) Chempakam, B; Kurup, P AItem Changes in some hormones by low doses of di (2-ethyl hexyl) phthalate (DEHP), a commonly used plasticizer in PVC blood storage bags & medical tubing.(2004-04-19) Gayathri, N S; Dhanya, C R; Indu, A R; Kurup, P ABACKGROUND & OBJECTIVES: Di (2-ethyl hexyl) phthalate (DEHP), a plasticizer commonly used in PVC blood storage bags leaches out in significant amounts into blood during storage. In view of many reports on the toxicity of this compound, it was considered necessary to investigate the effect of DEHP at the low level solubilized in blood on some important hormones in rats and in human blood stored in DEHP plasticized blood bags. METHODS: Rats were administered DEHP at a low level of 750 microg/100 g body weight on alternate days for 14 days. Changes in the serum insulin, blood glucose, liver glycogen level and T3, T4 and thyroid stimulating hormone (TSH) as well as cortisol in the serum were studied. Changes in the hormones were also studied in blood stored in DEHP plasticized PVC bags. RESULTS: The results indicated decrease in serum insulin, cortisol and liver glycogen, and increase in blood glucose, serum T3 and T4 in rats receiving DEHP. These changes were reversed when administration of DEHP was stopped. Similar changes in hormones were also observed in the blood stored in DEHP plasticized blood bags. INTERPRETATION & CONCLUSION: The results indicated that administration of DEHP at low levels to rats caused symptoms of diabetes, thyroid and adrenocortical dysfunction. Though the results obtained in rats cannnot be extrapolated to human, the fact that similar hormonal changes seen in human blood stored in DEHP plasticized blood bags may suggest possibility of DEHP causing similar changes in human. The fact that these changes were reversed in rats when DEHP administration was stopped, indicates that transfusion of a few units of blood to a recipient may not be harmful, but it may pose a problem during repeated transfusions such as in thalassaemia patients.Item Changes in the composition of erythrocyte membrane during storage of blood in di-(2-ethyl hexyl) phthalate [DEHP] plasticized poly vinyl chloride (PVC) blood storage bags.(1999-04-14) Manojkumar, V; Deepadevi, K V; Arun, P; Nair, K G; Lakshmi, L R; Kurup, P AVery little information is available on the changes in the erythrocyte membrane composition during storage of blood at 4 degrees C, particularly with respect to the glycosaminoglycans and glycoproteins. In view of this, a detailed study was carried out on the changes in the membrane proteins, glycosaminoglycans (GAG), carbohydrate components of glycoproteins, cholesterol, phospholipids and vitamin E in blood stored in glass bottles and a di-(2-ethyl hexyl) phthalate (DEHP) plasticized PVC bag (Penpol blood bag). Blood was collected in CPDA solution in glass bottles and in Penpol blood bags and kept at 4 +/- 1 degrees C. Analysis was made immediately after blood collection and after 28 and 42 days. Significant increase in the total protein in the erythrocyte membrane was observed during storage of whole blood in glass bottles and Penpol blood bag at 4 degrees C. This increase was progressively more with increase in storage time. Significant changes were also observed in GAG, carbohydrate components of glycoproteins, cholesterol, phospholipids and vitamin E in the erythrocyte membrane under these conditions. The protein:GAG ratio, protein:carbohydrate ratio, cholesterol:phospholipid ratio as well as protein:lipid ratio showed significant increase in the membrane. The extent of these changes was lower in the Penpol bag, indicating the stabilizing effect of DEHP on the erythrocyte membrane.Item Changes in the isoprenoid pathway in syndrome X.(2001-12-09) Kumar, A R; Kurup, P AThis study assessed the changes in digoxin and some other metabolites of the isoprenoid pathway in metabolic syndrome X presenting with multiple lacunar state. There was an increase in plasma HMG CoA reductase activity with a consequent increase in serum digoxin, which caused a reduction in RBC membrane Na+-K+ ATPase activity. There was an increase in serum tryptophan and its metabolites and a decrease in tyrosine and its metabolites. Serum magnesium was decreased with consequent alteration in the metabolism of glycosaminoglycans and glycolipids. Increase in dolichol, another product of the isoprenoid pathway resulted in alteration in glycoprotein metabolism. Changes in the composition of membrane glycosaminoglycans, glycoproteins and cholesterol:phospholipid ratio were also observed in this disorder leading to decreased lysosomal stability. Decrease in ubiquinone, another isoprenoid metabolite resulted in alteration in the free radical generation. Membrane Na+-K+ ATPase inhibition due to digoxin, altered membrane structure, increased tryptophan catabolites and decreased tyrosine catabolites can lead on to increased intracellular calcium and reduced intracellular magnesium which can account for the symptoms of syndrome X.