Purification of Colocasia esculenta lectin and determination of its anti-insect potential towards Bactrocera cucurbitae.
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Date
2013-01
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Abstract
The present study reports the purification of a lectin from Colocasia esculenta (L.) Schott corms and evaluation of its anti-insect potential towards Bactrocera cucurbitae (Coquilett). The lectin was found to be specific towards N-acetyl-D-lactosamine (LacNac), a disaccharide and asialofetuin, a desialylated serum glycoprotein in hemagglutination inhibition assay. Asialofetuin was used as a ligand to purify Colocasia esculenta agglutinin (CEA) by affinity chromatography. The purity of CEA was ascertained by the presence of a single band in reducing SDS-PAGE at pH 8.3. The affinity purified CEA was employed in artificial diet bioassay of second instar larvae (64-72 hr old) of the B. cucurbitae at concentrations ranging between 10-160 µg ml-1. The lectin significantly (p<0.01) decreased the percent pupation and emergence with respect to control. Effect on various enzymes was studied by employing LC50 (51.6 µg ml-1) CEA in the artificial diet bioassay of second instar larvae. All the enzymes tested namely esterases, phosphatases (acid and alkaline), superoxide dismutases, catalase and glutathione-S-transferase showed a significant (p<0.01, p<0.05) increase in their enzyme and specific activities. These results showed that CEA affected normal growth and development and presented stress to the larvae, activating their detoxification and anti-oxidant systems. Thus, the lectin seems to be a useful candidate for the control measures of B. cucurbitae under the integrated pest management (IPM) system.
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Keywords
Colocasia esculenta, Lectin, Bactrocera cucurbitae, Antioxidant enzymes, Hydrolytic enzymes, IPM
Citation
Thakur Kshema, Kaur Manpreet, Kaur Satwinder, Kaur Amritpal, Kamboj Sukhdev Singh, Singh Jatinder. Purification of Colocasia esculenta lectin and determination of its anti-insect potential towards Bactrocera cucurbitae. Journal of Environmental Biology. 2013 Jan; 34(1): 31-36.