Purification of Colocasia esculenta lectin and determination of its anti-insect potential towards Bactrocera cucurbitae.
| dc.contributor.author | Thakur, Kshema | |
| dc.contributor.author | Kaur, Manpreet | |
| dc.contributor.author | Kaur, Satwinder | |
| dc.contributor.author | Kaur, Amritpal | |
| dc.contributor.author | Kamboj, Sukhdev Singh | |
| dc.contributor.author | Singh, Jatinder | |
| dc.date.accessioned | 2013-09-24T10:41:07Z | |
| dc.date.available | 2013-09-24T10:41:07Z | |
| dc.date.issued | 2013-01 | |
| dc.description.abstract | The present study reports the purification of a lectin from Colocasia esculenta (L.) Schott corms and evaluation of its anti-insect potential towards Bactrocera cucurbitae (Coquilett). The lectin was found to be specific towards N-acetyl-D-lactosamine (LacNac), a disaccharide and asialofetuin, a desialylated serum glycoprotein in hemagglutination inhibition assay. Asialofetuin was used as a ligand to purify Colocasia esculenta agglutinin (CEA) by affinity chromatography. The purity of CEA was ascertained by the presence of a single band in reducing SDS-PAGE at pH 8.3. The affinity purified CEA was employed in artificial diet bioassay of second instar larvae (64-72 hr old) of the B. cucurbitae at concentrations ranging between 10-160 µg ml-1. The lectin significantly (p<0.01) decreased the percent pupation and emergence with respect to control. Effect on various enzymes was studied by employing LC50 (51.6 µg ml-1) CEA in the artificial diet bioassay of second instar larvae. All the enzymes tested namely esterases, phosphatases (acid and alkaline), superoxide dismutases, catalase and glutathione-S-transferase showed a significant (p<0.01, p<0.05) increase in their enzyme and specific activities. These results showed that CEA affected normal growth and development and presented stress to the larvae, activating their detoxification and anti-oxidant systems. Thus, the lectin seems to be a useful candidate for the control measures of B. cucurbitae under the integrated pest management (IPM) system. | en_US |
| dc.identifier.citation | Thakur Kshema, Kaur Manpreet, Kaur Satwinder, Kaur Amritpal, Kamboj Sukhdev Singh, Singh Jatinder. Purification of Colocasia esculenta lectin and determination of its anti-insect potential towards Bactrocera cucurbitae. Journal of Environmental Biology. 2013 Jan; 34(1): 31-36. | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/148487 | |
| dc.language.iso | en | en_US |
| dc.source.uri | https://www.jeb.co.in/journal_issues/201301_jan13/paper_05.pdf | en_US |
| dc.subject | Colocasia esculenta | en_US |
| dc.subject | Lectin | en_US |
| dc.subject | Bactrocera cucurbitae | en_US |
| dc.subject | Antioxidant enzymes | en_US |
| dc.subject | Hydrolytic enzymes | en_US |
| dc.subject | IPM | en_US |
| dc.title | Purification of Colocasia esculenta lectin and determination of its anti-insect potential towards Bactrocera cucurbitae. | en_US |
| dc.type | Article | en_US |