Production of Fungal Pectin Lyase and Polygalacturonase from Fruit Wastes by Solid State Fermentation
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Date
2022-08
Journal Title
Journal ISSN
Volume Title
Publisher
Sciencedomain International
Abstract
Polygalacturonase (PG or PGase) and Pectin lyase (PL) are depolymerase enzymes that split the ?-1, 4-glycosidic linkages in the backbone of homogalacturonans. They are produced by microorganisms degrading pectin-containing substrates. PG and PL were produced in the solid-state fermentation (SSF) of beans testa (BT), mango peels (MP), Plantain peels (PP), and BT: MP: PP (1:1:1). Substrates were seeded with individual fungal strains namely Aspergillus tamarii, Aspergillus terreus, Aspergillus piperis, Aspergillus parasiticus and Mucor piriformis. PG production ranged from 0.0377 - 141.0095 U/g with A. tamarii, A. terreus and A. piperis producing the highest on mango peels. PL production ranged from 50.50 - 10,852.50 U/g with Mucor piriformis producing the highest on plantain peels. The pH of the fermentation medium changed during growth, metabolism, and pectinase production. The best pH for pectinase production falls within the acidic range. Unconventional substrates such as PP are viable for pectinase production, and PL yield in SSF is improved by compositing substrates.
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Keywords
Fungi, polygalacturonase, pectin lyase, fruit waste, solid state fermentation.Original ResearchArticle
Citation
Amande T. J., Ado B. V., Adebayo-Tayo B. C.. Production of Fungal Pectin Lyase and Polygalacturonase from Fruit Wastes by Solid State Fermentation. Biotechnology Journal International . 2022 Aug; 26(4): 48-56