Transforming growth factor-β-1 polymorphisms are infrequent but exist at selected loci in oral submucous fibrosis.

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dc.contributor.authorRajendran, R
dc.contributor.authorHarish, R K
dc.contributor.authorAnil, Sukumaran
dc.contributor.authorVidyadharan, Ravi
dc.contributor.authorBanerjee, Moinak
dc.date.accessioned2012-07-27T03:14:19Z
dc.date.available2012-07-27T03:14:19Z
dc.date.issued2010-07
dc.description.abstractBackground : Oral submucous fibrosis (OSF) may be considered a collagen metabolic disorder resulting from areca-nut alkaloid exposure and individual variation in collagen metabolism. Due to the complexity of OSF pathogenesis, it is important to elucidate independent and interactive effects of polymorphisms of collagen-related genes on OSF risk. Materials and Methods : This study is focused on seven polymorphisms (SNPs) of transforming growth factor-beta-1 (TGF-beta-1) gene in patients with oral submucous fibrosis (OSF), belonging to south Indian ethnic extraction. The mean age at presentation was 43.9 years, range 23-72 years (n=50, M:F ratio, 2.6:1). DNA samples from 50 subjects of the same ethnic group and comparable demographic features who have had practiced the habit of areca-chewing of almost equal duration, but remained free of disease constituted the controls. All DNA samples were collected progressively and purified from peripheral blood employing standard protocols and tested for SNPs. They included two polymorphisms in the promoter region (C-509T and G-800A), three polymorphisms in exon-1 (Arg25Pro(G915C), Leu10Pro(T869C), Glu47Gly(A979G) and two in 5 ͲUTR regions (C→T(rs13306708) and G→A (rs9282871). The extracted DNA samples along with the primers underwent PCR amplification and the genotypic and allelic frequencies were calculated. All calculations were performed using the SPSS software. The PCR products were purified and subsequently sequenced using Flour S™ multi-imager system (Biorad). The sequenced data were analyzed using the BioEdit sequence analysis software. Results : Out of the seven polymorphisms analyzed, six such as two in the promoter region, three in exon-1 and one in 5¢UTR were found to have a " P" value above 0.05 and hence were not significant. The C→T transition (rs13306708) in the 5¢UTR region recorded a " P" value of 0.03 on comparison and hence was found to be significant. The allelic frequencies for this C→T transition in patients were 68.7% C and 31.2% T (27CC, 15CT, 8TT) and that in controls were 89.5% C and 10.4% T (42CC, 6CT, 2TT). Conclusions : The polymorphism in 5¢UTR C-T in TGF beta 1 gene has a significant association with OSF, being a prime determinant in the pro-angiogenic pathway which has got direct bearing with the pathophysiology of the disease. The proximity of this polymorphism to the transcription site and the associated risk involved is discussed.en_US
dc.identifier.citationRajendran R, Harish R K, Anil Sukumaran, Vidyadharan Ravi, Banerjee Moinak. Transforming growth factor-β-1 polymorphisms are infrequent but exist at selected loci in oral submucous fibrosis. Indian Journal of Dental Research. 2010 Jul-Sept; 21(3): 413-419.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/139892
dc.language.isoenen_US
dc.source.urihttps://www.ijdr.in/article.asp?issn=0970-9290;year=2010;volume=21;issue=3;spage=413;epage=419;aulast=Rajendranen_US
dc.subjectCytokinesen_US
dc.subjectfibrogenesisen_US
dc.subjectoral submucous fibrosisen_US
dc.subjectTGF-b1en_US
dc.subjectSNPsen_US
dc.subject.mesh5' Untranslated Regions --genetics
dc.subject.meshAdenine
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshAreca
dc.subject.meshArginine --genetics
dc.subject.meshChromosome Mapping
dc.subject.meshCytosine
dc.subject.meshEthnic Groups --genetics
dc.subject.meshExons --genetics
dc.subject.meshFemale
dc.subject.meshGene Frequency --genetics
dc.subject.meshGenotype
dc.subject.meshGlutamine --genetics
dc.subject.meshGlycine --genetics
dc.subject.meshGuanine
dc.subject.meshHumans
dc.subject.meshIndia
dc.subject.meshLeucine --genetics
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshOral Submucous Fibrosis --genetics
dc.subject.meshOral Submucous Fibrosis --immunology
dc.subject.meshPolymorphism, Single Nucleotide --genetics
dc.subject.meshProline --genetics
dc.subject.meshPromoter Regions, Genetic --genetics
dc.subject.meshThymine
dc.subject.meshTime Factors
dc.subject.meshTransforming Growth Factor beta1 --genetics
dc.subject.meshYoung Adult
dc.titleTransforming growth factor-β-1 polymorphisms are infrequent but exist at selected loci in oral submucous fibrosis.en_US
dc.typeArticleen_US
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