Detection of superoxide dismutase isozymes in normal and X-irradiated chick amniotic fluid by using isoelectric focussing.

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dc.contributor.authorSarala, Pen_US
dc.contributor.authorSuneetha, Sen_US
dc.contributor.authorParthasarathy, P Ren_US
dc.date.accessioned2009-05-28T16:33:45Z
dc.date.available2009-05-28T16:33:45Z
dc.date.issued1996-09-01en_US
dc.description.abstractIsoelectric focussing of amniotic fluid of chick system over pH gradient of 4-6 revealed presence of 8 superoxide dismutases. The superoxide dismutase (SOD) isozymes were identified as one major isozyme and other seven were minor ones. Higher expression of SOD (major isozyme) with exposure of 1000 R was studied. The isoelectric point (pI) of major SOD isozyme in control (5) differs from radiation exposed samples (5.12). Hence in radiation exposed amniotic fluid showed more pronounced SOD major isozyme, than control amniotic fluid. It is clearly evident that the SOD isozyme with X-irradiation having different isoelectric points, may lead to modification of the amino acid composition and charges of amino acids in the protein structure of SOD. So, the higher SOD isozyme expression has a role in defence action against free radical damage by X-irradiation during embryonic development.en_US
dc.description.affiliationDepartment of Biochemistry, School of Biological and Earth Sciences, Sri Venkateswra University, Tirupati, India.en_US
dc.identifier.citationSarala P, Suneetha S, Parthasarathy PR. Detection of superoxide dismutase isozymes in normal and X-irradiated chick amniotic fluid by using isoelectric focussing. Indian Journal of Experimental Biology. 1996 Sep; 34(9): 901-4en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/63388
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAmniotic Fluid --enzymologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChick Embryoen_US
dc.subject.meshIsoelectric Focusingen_US
dc.subject.meshIsoenzymes --metabolismen_US
dc.subject.meshSuperoxide Dismutase --metabolismen_US
dc.subject.meshX-Raysen_US
dc.titleDetection of superoxide dismutase isozymes in normal and X-irradiated chick amniotic fluid by using isoelectric focussing.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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