Attachment of spermatozoa to in vitro matured bovine oocytes in TCM-199 and MEM media.

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dc.contributor.authorSahoo, T Ken_US
dc.contributor.authorMohanty, D Nen_US
dc.contributor.authorMohanty, B Nen_US
dc.contributor.authorBarik, A Ken_US
dc.date.accessioned2009-05-28T14:39:20Z
dc.date.available2009-05-28T14:39:20Z
dc.date.issued1998-04-26en_US
dc.description.abstractInvestigation was undertaken to study the efficacy of TCM 199 and MEM media in the process of oocyte maturation and fertilization after harvesting the oocytes from slaughtered cow and heifer ovaries by aspiration and mincing techniques. Out of 354 number of ovaries, mincing method could yield 1.35 (50.98%) of good quality oocytes per ovary as comparison to 0.61 (55.45%) by aspiration method. The TCM 199 and MEM could record the maturation of 85.71% and 60.26% of oocytes and the rate of sperm penetration was higher in TCM 199 matured oocytes (97.21%) as compared to MEM matured ones (74.46%). The staining of oocytes by aceto-orcein stain was also employed.en_US
dc.description.affiliationDepartment of Gynaecology, Faculty of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar.en_US
dc.identifier.citationSahoo TK, Mohanty DN, Mohanty BN, Barik AK. Attachment of spermatozoa to in vitro matured bovine oocytes in TCM-199 and MEM media. Indian Journal of Experimental Biology. 1998 Apr; 36(4): 367-70en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/60989
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCattleen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshFemaleen_US
dc.subject.meshFertilization in Vitroen_US
dc.subject.meshMaleen_US
dc.subject.meshOocytes --cytologyen_US
dc.subject.meshSperm-Ovum Interactionsen_US
dc.subject.meshSpermatozoa --cytologyen_US
dc.titleAttachment of spermatozoa to in vitro matured bovine oocytes in TCM-199 and MEM media.en_US
dc.typeJournal Articleen_US
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