Evaluation of invitro antidiabetic activity using silver nanoparticles of Galinsoga parviflora leaf extract
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Date
2024-10
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Medip Academy
Abstract
Background: Diabetes mellitus, characterized by chronic hyperglycemia, can lead to severe complications if untreated. G. parviflora, a plant from the Astereaceae family, is traditionally recognized for its medicinal properties, including hypoglycemic effects. This study investigates the antidiabetic potential of silver nanoparticles synthesized from G. parviflora leaf extract. To evaluate the in-vitro antidiabetic activity of silver nanoparticles from G. parviflora by assessing their ?-amylase and ?-glucosidase inhibitory activities, and glucose uptake using the everted gut sac method. Methods: Silver nanoparticles were synthesized from G. parviflora and tested for ?-amylase and ?-glucosidase inhibitory effects using colorimetric methods. Glucose uptake activity was assessed with an everted gut sac model from chicken ileum. Statistical analysis employed one-way ANOVA and Tukey’s post-hoc test, with significance set at p<0.05. Results: The nanoparticles exhibited significant ?-amylase inhibitory activity, with 90.2% inhibition at 200 µl and 91.9% at 400 µl, surpassing the standard drug Acarbose. They also showed dose-dependent ?-glucosidase inhibitory activity, achieving 34.5% inhibition at 200 µl and 32.6% at 400 µl. Glucose uptake analysis revealed significant reductions at 150 minutes for both the standard drug and nanoparticles compared to the control (p<0.05). However, at 300 minutes, only the standard treatment maintained significant differences. Conclusions: Silver nanoparticles of G. parviflora exhibit significant in vitro antidiabetic activity by inhibiting key digestive enzymes and reducing glucose uptake. These findings suggest their potential for developing novel antidiabetic therapies.
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Department of Pharmacology, Sri Adichunchangiri College of Pharmacy, Rajiv Gandhi University of Health Sciences, Bengaluru, Karnataka, India
Citation
Bhat WH, Ahmed SS.. Evaluation of invitro antidiabetic activity using silver nanoparticles of Galinsoga parviflora leaf extract . International Journal of Basic & Clinical Pharmacology. 2024 Oct; 13(5): 629-635