Purification and characterization of buffalo liver L-arginase and its kinetic properties with dihydropyrimidine and metal ions.

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dc.contributor.authorNile, Shivraj Hariram
dc.contributor.authorPark, Se Won
dc.date.accessioned2016-11-23T10:41:11Z
dc.date.available2016-11-23T10:41:11Z
dc.date.issued2016-06
dc.description.abstractArginase (L-arginine amidinohydrolase, EC.3.5.3.1) from animal tissues such as, liver and kidney has been partially characterized by many researchers. In this study, we purified arginase to homogeneity from buffalo liver with about ~2857 purification fold and a 20% recovery by chromatographic and spectroscopic analysis were obtained. The molecular mass determined by gel filtration and SDS-PAGE was found to be 118 kDa and 47 kDa, respectively. The optimal pH and temperature of the arginase was 9.5 and 40°C, respectively. Kinetic parameters (Km and Vmax) showed activation of arginase in the reaction medium with decrease in Km (7.14, 5.26, 4.0 and control 3.22 mM) and Vmax (0.05, 0.035, 0.027 and control 0.021 mg/mL/min), while co-factor activity of arginase was optimized using metal ions like Mn2+ and Mg2+ at 2 mM, which revealed an increase in Vmax values (0.011, 0.013, 0.015 and control 0.010 mg/mL/min) and a decrease in Km values (2.22, 2.12, 1.88 and control 1.66 mM). The kinetic data suggested that the arginase activity is enhanced in the presence of dihydropyrimidine derivative and metal ions, indicating essential mode of activation.en_US
dc.identifier.citationNile, Shivraj Hariram Park, Se Won. Purification and characterization of buffalo liver L-arginase and its kinetic properties with dihydropyrimidine and metal ions. Indian Journal of Experimental Biology. 2016 June; 54(6): 414-419.en_US
dc.identifier.issn0975-1009
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/178764
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/34327en_US
dc.subjectDihydropyrimidine derivativeen_US
dc.subjectIsoenzymesen_US
dc.subjectNitrotelismen_US
dc.subjectPyrimidinesen_US
dc.subjectUreogenesisen_US
dc.titlePurification and characterization of buffalo liver L-arginase and its kinetic properties with dihydropyrimidine and metal ions.en_US
dc.typeArticleen_US
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