Isolation and purification of cuticle degrading extra cellular proteases from entomopathogenic fungal species of beauveria bassiana and metarhizium anisopliae.

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dc.contributor.authorLakshmi, S Bhagya
dc.contributor.authorKaur, S Gurvinder
dc.contributor.authorPalem, P C Padmini
dc.date.accessioned2015-08-03T11:14:37Z
dc.date.available2015-08-03T11:14:37Z
dc.date.issued2010-11
dc.description.abstractEnhanced Pr1 and Pr2 activities were observed in entomopathogenic fungi Beauveria bassiana (UB9) and Metarhizium anisopliae (UM4) supplemented with casein. Highest activity was observed at 72 hours of incubation when compared to minimal medium (MM) and colloidal chitin amended media. The extra cellular proteases (Pr1 and Pr2) thus isolated were purified through dialysis and their activities were found to be increased by two-fold. Pr1 and Pr2 were subjected to SDS - PAGE analysis and activity gel electrophoresis. The molecular weight of protease produced by Beauveria bassiana (UB9) is 19 KD and that of Metarhizium anisopliae (UM4) is 21 KD.en_US
dc.identifier.citationLakshmi S Bhagya, Kaur S Gurvinder, Palem P C Padmini. Isolation and purification of cuticle degrading extra cellular proteases from entomopathogenic fungal species of beauveria bassiana and metarhizium anisopliae. International Journal of Applied Biology and Pharmaceutical Technology. 2010 Nov-Dec; 1(3): 1150-1156.en_US
dc.identifier.issn0976-4550
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/161189
dc.language.isoenen_US
dc.source.urihttps://www.ijabpt.com/Details.aspx?fid=154en_US
dc.subjectExtracellular proteasesen_US
dc.subjectPr1en_US
dc.subjectPr2en_US
dc.subjectBeauveria bassianaen_US
dc.subjectMetarhizium anisopliaeen_US
dc.titleIsolation and purification of cuticle degrading extra cellular proteases from entomopathogenic fungal species of beauveria bassiana and metarhizium anisopliae.en_US
dc.typeArticleen_US
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