Cytotoxic, Thrombolytic and Membrane Stabilizing Activities of Swietenia mahagoni (L.) Jacq. Flower Extract.
Loading...
Date
2014-10
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aims: To investigate cytotoxic, thrombolytic and membrane stabilizing activities of
methanol extract and its different Kupchan partitionates of flowers of Swietenia mahagoni.
Study Design: Evaluation of cytotoxic activity using brine shrimp nauplii, thrombolytic and
membrane stabilizing activities on human RBCs.
Place and Duration of Study: Phytochemical Research Laboratory, Department of
Pharmacy, School of Health Science, State University of Bangladesh, from April to
September, 2013.
Methodology: The eggs of brine shrimp nauplii were hatched in artificial sea water for 24
hours. Cytotoxic activity was determined by measuring the percentage of their mortality
after application of different partitionates to them. Human RBCs were obtained from 3 healthy volunteers. Thrombolytic activity was calculated by weighing the clot before and
after addition of different partitionates while membrane stabilizing activity was evaluated
in terms of inhibition of percentage of haemolysis of RBCs by measuring optical density in
both hypotonic and heat induced conditions.
Results: The highest cytotoxic activity was achieved with the crude methanol extract
(LC50 = 0.10±0.01 mg/ml) among the partitionates while vincristine sulfate, the positive
control, achieved an LC50 value of 0.40±0.02 mg/ml. While investigating thrombolytic
activity, the petroleum ether soluble fraction achieved the highest clot lysis activity
(34.30±0.78%) compared to the standard streptokinase (70.27±1.26%). While
determining the membrane stabilizing activity, in hypotonic solution induced condition, the
crude methanol extract inhibited 84.71±3.25% haemolysis of RBCs whereas in heat
induced condition, the aqueous soluble fraction inhibited 86.30±4.98% haemolysis of
human RBCs. Here, acetyl salicylic acid (0.01mg/ml) used as reference standard showed
71.91±2.29% and 45.45±4.87% inhibition of haemolysis of human RBCs in hypotonic
solution and heat induced conditions, respectively.
Conclusion: From our investigation, it can be suggested that, the flower extractives can
further be studied extensively to find out their efficacy.
Description
Keywords
Swietenia mahagoni, flowers, brine shrimp lethality, hrombolytic, membrane, stabilizing, hypotonic solution
Citation
Rahman S M Ashikur, Akter Maksuda, Hira Tasnuva Easmin, Sharmin Tasnuvac, Nayeen Md Junayed. Cytotoxic, Thrombolytic and Membrane Stabilizing Activities of Swietenia mahagoni (L.) Jacq. Flower Extract. European Journal of Medicinal Plants. 2014 Oct; 4(10): 1232-1239.