Characterization and metabolomic analysis of Plant-derived Extracellular Vesicles (PdEVs) isolated from indigenous medicinal plants
No Thumbnail Available
Date
2024-12
Journal Title
Journal ISSN
Volume Title
Publisher
Open Science Publishers LLP
Abstract
PdEVs (Plant-derived Extracellular Vesicles) are nano-sized, membranous vesicles released by plant cells for defense mechanisms. They contain nucleic acids, proteins, lipids, and other secondary metabolites. In the current study, we characterized PdEVs derived from medicinal plants and identified the metabolites present in the PdEVs. We isolated PdEVs using the PROSPR method from the apoplastic fluid of four different medicinal plants: Azadirachta indica, Murraya koenigii, Ocimum tenuiflorum, and Coleus amboinicus. The isolated PdEVs were characterized morphologically by HR-TEM, and functional group characterization was performed using FTIR. Furthermore, metabolomic analysis was performed using GC-MS for the PdEVs from Azadirachta indica, Murraya koenigii, Ocimum tenuiflorum, and Coleus amboinicus. We found the identified metabolites to possess anti-cancer, anti- microbial, and anti-fungal activities, substantiating the potential use of PdEVs as a potential source for therapeutic applications.
Description
Keywords
Extracellular vesicles, Metabolomics, Medicinal plants, GC-MS, FTIR.
Citation
Ravitchandirane H, Singh V, Rajavel A, Sella RN. . Characterization and metabolomic analysis of Plant-derived Extracellular Vesicles (PdEVs) isolated from indigenous medicinal plants. Journal of Applied Biology & Biotechnology. 2024 Dec; 12(6): 251-260