The histology of the pineal gland in cadaveric embalmed specimens.

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dc.contributor.authorUrusopon, Kriengkrai
dc.contributor.authorPhornkunnatham, Umphaphorn
dc.contributor.authorRoongruangchai, Jantima
dc.contributor.authorSripaoraya, Kesorn
dc.contributor.authorViravud, Yadaridee
dc.date.accessioned2012-05-23T07:41:08Z
dc.date.available2012-05-23T07:41:08Z
dc.date.issued2012-01
dc.description.abstractObjective: This study is to observe ultrastructurally of the pineal gland from cadaveric embalmed specimens by light microscopy (LM) and transmission electron microscopy (TEM) Methods: Ten pineal glands were removed from cadaveric embalmed specimens. Each pineal gland was disected into two groups, one was put into 10% formaldehyde and the other was put into 2.5% glutaraldehyde. The first group was processed for light microscopy. First of all, fixed the tissues in formalin and then embedded in paraffin. Next, serially sectioned at eight micron and finally stained. Staining methods were (i) haematoxylin and eosin, (ii) Masson-Fontana method for melanin. The second group well preserved in 2.5% glutaraldehyde was chosen to prepare for the TEM. Results: Only five out of ten cadaveric pineal glands viewed by light microscopy were well preserved. In LM, we saw clearly that all ultrastructures or morphology of the cadaveric embalmed pineal gland cells were the same as the standard textbook. Melanin pigments were accumulated in both of the cytoplasm of pinealocytes and the stroma of pineal gland proved by Masson-Fontana staining. Mast cells were found throughout the gland but preferably found in the connective tissue trabeculae. A neuronal-like cell was found in the parenchyma of pineal gland. Extrapineal and intrapineal calcified concretion called corpora arenacea or brain sand were presented. Intrapineal concretions in the parenchyma were globular and concentric lamellar patterns while extrapineal concretions which were adjacent to the capsule were concentric lamellar only. TEM of pineal gland showed that it was moderately preserved in the chemical fixative of this formula. Cadaveric pinealocyte showed some organelles and chromatin extraction while the environmental fibrillar structures were well preserved. Conclusion: The histological findings in the pineal glands from cadaveric embalmed specimens are similar to fresh cadavers. Moreover, they can be used to prepare the normal slides for Histology Lab of the second year medical students. The presences of the melanin pigments in the cytoplasm of pinealocytes and stroma, mast cells, corpora arenacea, and neuronal-like cells confirm the previous studies.en_US
dc.identifier.citationUrusopon Kriengkrai, Phornkunnatham Umphaphorn, Roongruangchai Jantima, Sripaoraya Kesorn, Viravud Yadaridee. The histology of the pineal gland in cadaveric embalmed specimens. Siriraj Medical Journal, 2012 Jan; 64(suppl): 54-57.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/138584
dc.language.isoenen_US
dc.source.urihttps://www.sirirajmedj.com/content_download.php?content_id=2644en_US
dc.subjectPineal glanden_US
dc.subjectlight microscopyen_US
dc.subjectmelanin pigmentsen_US
dc.subjectcorpora arenaceaen_US
dc.titleThe histology of the pineal gland in cadaveric embalmed specimens.en_US
dc.typeArticleen_US
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