Homologous radioimmunoassay and radioreceptorassay of gonadotropic hormone for an Indian carp Catla catla.

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dc.contributor.authorManna, P Ren_US
dc.contributor.authorBanerjee, P Pen_US
dc.contributor.authorBhattacharya, Sen_US
dc.date.accessioned2009-05-28T12:31:34Z
dc.date.available2009-05-28T12:31:34Z
dc.date.issued1989-05-01en_US
dc.description.abstractA homologous radioimmunoassay (RIA) for Indian carp (C. catla) gonadotropin (GtH) was developed by using purified Catla GtH and its specific antiserum. In Ouchterlony agar diffusion, antisera raised against purified Catla GtH (cGtH), showed clear crossreaction. Catla-anti GtH (anti-cGtH) did not cross-reacted with Catla TSH enriched fraction. Immunocrossreaction was further confirmed with competitive binding inhibition studies where displacement of radiolabelled cGtH was precisely linear against increasing concentrations of cGtH, hence this was later used as standard curve for RIA. Competitive binding inhibition was also observed with purified murrel GtH, silver carp GtH and Cyprinus GtH, using varied doses. Both murrel and silver carp GtH showed clear parallelism with cGtH, while Cyprinus GtH inhibition slope was less steeper. Mammalian GtHs (hCG, oLH, oFSH), bTSH and bPRL had no crossreaction with anti-cGtH. Radioreceptorassay (RRA) for cGtH was developed by preparing plasma membranes from Catla oocytes. Binding of 125I-cGtH to oocyte plasma membranes showed saturability with high affinity (Ka = 0.11 X 10(13)M-1) and low capacity (17 fmol/mg protein). Displacement of 125I-cGtH in receptorassay by cold cGtH was linear and therefore served as standard curve. The interassay and intrassay variability in RIA was 7.9% and 3% while that of RRA was 5% and 3% respectively. Sensitivity of RIA was in the picogram level whereas it was in nanogram level by RRA. Determination of carp pituitary and serum GtH content by RIA and RRA showed the consistency, precision and validity of these assays. Although RRA was comparatively less sensitive than RIA, it was convenient, quick and less expensive.en_US
dc.description.affiliationDepartment of Zoology, School of Life Science, Visva-Bharati University, Santiniketan 731 235, India.en_US
dc.identifier.citationManna PR, Banerjee PP, Bhattacharya S. Homologous radioimmunoassay and radioreceptorassay of gonadotropic hormone for an Indian carp Catla catla. Indian Journal of Experimental Biology. 1989 May; 27(5): 399-403en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/58201
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCarps --blooden_US
dc.subject.meshFemaleen_US
dc.subject.meshGonadotropins --blooden_US
dc.subject.meshPerciformes --blooden_US
dc.subject.meshRadioimmunoassayen_US
dc.subject.meshRadioligand Assayen_US
dc.titleHomologous radioimmunoassay and radioreceptorassay of gonadotropic hormone for an Indian carp Catla catla.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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