Sperm motility inhibiting activity of a phytosterol from Alstonia macrophylla Wall ex A. DC. leaf extract: a tribal medicine.

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dc.contributor.authorChattopadhyay, Debprasaden_US
dc.contributor.authorDungdung, Sandhya Ren_US
dc.contributor.authorDas, Kaushiken_US
dc.contributor.authorSaha, Sudiptaen_US
dc.contributor.authorMandal, Asit Ben_US
dc.contributor.authorMajumder, Gopal Cen_US
dc.date.accessioned2009-05-28T14:47:00Z
dc.date.available2009-05-28T14:47:00Z
dc.date.issued2005-11-30en_US
dc.description.abstractThe role of methanolic extract and n-butanol fraction of A. macrophylla leaves was investigated on the forward motility of goat spermatozoa. The methanol extract (600 micro/g/ml) and one n-butanol fraction (Fraction A; 100 microg/ml) showed marked inhibition of sperm forward motility, tested by microscopic and spectrophotometric methods. Approximately, 50-60% of the spermatozoa lost their motility when treated with 600 microg/ml of methanol extract or 100 microg/ml of Fraction A. The Fraction A at 400 microg/ml concentration showed complete inhibition of sperm forward motility at 0 min. The inhibitory activity increased with the increasing concentrations of the fraction. The motility inhibitory activity of the Fraction A was stable to heat treatment at 100 degrees C for 2 min. The compound showed high inhibitory effect in the pH range 6.7-7.6. Fraction A also showed high efficacy for inhibiting human sperm motility, assessed by the microscopic method. The phytochemical analysis of methanolic extract of A. macrophylla leaves revealed the presence of sterols, triterpene, flavonoid, alkaloid, tannin and reducing sugar, while the Fraction A contains beta-sitosterol, a common phytosterol. The results demonstrate that Fraction A (beta-sitosterol) is a potent inhibitor of sperm motility and thus it has the potential to serve as a vaginal contraceptive.en_US
dc.description.affiliationICMR Virus Unit, Infectious Diseases & Beliaghata General Hospital, General Block 4, 1st Floor, 57 Dr. Suresh Chandra Banerjee Road, Kolkata 700 010, India.en_US
dc.identifier.citationChattopadhyay D, Dungdung SR, Das K, Saha S, Mandal AB, Majumder GC. Sperm motility inhibiting activity of a phytosterol from Alstonia macrophylla Wall ex A. DC. leaf extract: a tribal medicine. Indian Journal of Experimental Biology. 2005 Nov; 43(11): 1104-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/61151
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAlstonia --metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnti-Infective Agents --pharmacologyen_US
dc.subject.meshAntifungal Agents --pharmacologyen_US
dc.subject.meshButanols --chemistryen_US
dc.subject.meshContraceptive Agents --pharmacologyen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshFemaleen_US
dc.subject.meshGoatsen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshMaleen_US
dc.subject.meshMedicine, Traditionalen_US
dc.subject.meshMethanol --chemistryen_US
dc.subject.meshPhytosterols --chemistryen_US
dc.subject.meshPlant Extracts --metabolismen_US
dc.subject.meshPlant Leaves --metabolismen_US
dc.subject.meshSitosterols --chemistryen_US
dc.subject.meshSperm Motility --drug effectsen_US
dc.subject.meshSpermatozoa --drug effectsen_US
dc.subject.meshTime Factorsen_US
dc.titleSperm motility inhibiting activity of a phytosterol from Alstonia macrophylla Wall ex A. DC. leaf extract: a tribal medicine.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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