Antifertility activity of Oroxylum indicum (L.) Kurz:In vitro and in vivo study on human sperm and male wistar rats
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Date
2023-01
Journal Title
Journal ISSN
Volume Title
Publisher
CSIR-NIScPR
Abstract
Oroxylum indicum (L.) Kurz, commonly called as Broken bones tree or Indian trumpet flower, belonging to Fam. Bignoniaceae, is traditionally used as a contraceptive by ethnic people of Tripura, North-East India. Here, we investigated the scientific basis for use of O. indicum as male antifertility agent by folklore healers. In vitro spermicidal activity of aqueous (AEOI) and methanolic (MEOI) extracts of O. indicum stem bark were studied on human sperm. The in vivo activity was experimented on male albino rats. The treated animals were allowed to mate and the pups delivered by female rat partners were counted. Phytochemical estimation of test samples was done using HPLC. The AEOI and MEOI treatments significantly decreased human sperm motility and viability. Test extracts have increased the hypo-osmotic swelling of sperm. Both the extracts were significantly declined the weight of reproductive organ. The MEOI treated rats have shown significant decrease in sperm motility and sperm counts. AEOI and MEOI treatment significantly reduced level of testosterone, but sharply raised dihydrotestosterone and prostaglandin in rats. Results testified the traditional claim for use of O. indicum as a male contraceptive agent, where MEOI have shown reversible action on male reproductive system leading to contraception without harming the libido.
Description
Keywords
Baicalin, Broken bones tree, Contraceptive, Indian trumpet flower, Kampong, Oroxylin, Sperm motility, Testosterone
Citation
Das Banani, Roy Kalyan, Talukdar Anupam Das, Dey Amitabha, Nongalleima Khumukcham, Das Supriya, Sharma Prasanti, Choudhury Manabendra Dutta, Deb Lokesh. Antifertility activity of Oroxylum indicum (L.) Kurz:In vitro and in vivo study on human sperm and male wistar rats. Indian Journal of Experimental Biology. 2023 Jan; 61(1): 33-41