Quantification of Stigmasterol in Successive Extracts of Clerodendrum Serratum, Poly Herbal Formulation By Hptlc Method And In-vitro Anti - Oxidant Activity Studies.

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dc.contributor.authorJayaprakasam, R
dc.contributor.authorRamakrishnan, M
dc.contributor.authorJyothi, B Asha
dc.contributor.authorSukumar, M
dc.contributor.authorRavi, T K
dc.date.accessioned2013-11-18T05:39:09Z
dc.date.available2013-11-18T05:39:09Z
dc.date.issued2013-10
dc.description.abstractThe aim of the present study is to carry out the quantification of Clerodendrum serratum containing poly herbal formulations by HPTLC and to study the invitro anti-oxidant activity of nitric oxide scavenging activity of the roots of Clerodendrum serratum.The rhizome part of Clerodendrum serratum has been selected, dried, powdered and extracted with petroleum ether, chloroform, ethyl acetate and methanol successively by continuous hot percolation method using soxhlet apparatus. Preliminary phytochemical screening of the successive extracts showed the presence of chemical constituents like triterpenoids, flavonoids, alkaloids, tannins, glycosides and sesquiterpenes. The Rf value of stigmasterol was found to be 0.80 ± 0.02.The developed methods were validated as per ICH guidelines. The LOD and LOQ was found to be 100 and 300 ng/spot respectively for HPTLC. The anti-oxidant activity was highest in petroleum ether extract when compared to the other extracts by using ascorbic acid as control. This shows that the polyherbal formulation containing stigmasterol has medicinal value and that it may be utillised for its anti-oxidant properties.en_US
dc.identifier.citationJayaprakasam R, Ramakrishnan M, Jyothi B Asha, Sukumar M, Ravi T K. Quantification of Stigmasterol in Successive Extracts of Clerodendrum Serratum, Poly Herbal Formulation By Hptlc Method And In-vitro Anti - Oxidant Activity Studies. International Journal of Research and Development in Pharmacy and Life Sciences. 2013 Oct-Nov; 2(6): 726-733.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/149360
dc.language.isoenen_US
dc.source.urihttps://www.ijrdpl.com/docs/v2i6/Microsoft%20Word%20-%2015.pdfen_US
dc.subjectClerodendrum serratumen_US
dc.subjectstigmasterolen_US
dc.subjectHPTLCen_US
dc.subjectascorbic aciden_US
dc.titleQuantification of Stigmasterol in Successive Extracts of Clerodendrum Serratum, Poly Herbal Formulation By Hptlc Method And In-vitro Anti - Oxidant Activity Studies.en_US
dc.typeArticleen_US
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