Genomic Analysis of Fungal Species No.11243 Mutant Strains Provides Insights into the Relationship between Mutations and High Productivity.

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dc.contributor.authorMatsui, Makoto
dc.contributor.authorYokoyama, Tatsuya
dc.contributor.authorKumagai, Toshitaka
dc.contributor.authorNemoto, Kaoru
dc.contributor.authorTerai, Goro
dc.contributor.authorMachida, Masayuki
dc.contributor.authorShibata, Takashi
dc.contributor.authorAburatani, Sachiyo
dc.date.accessioned2016-12-19T06:36:13Z
dc.date.available2016-12-19T06:36:13Z
dc.date.issued2016
dc.description.abstractAims: FR901469 is a novel antifungal antibiotic produced by fungal species No.11243. Although we have several FR901469 high-producing mutant strains, the mechanism of high productivity is unclear. This study aims to unravel the relationship between mutations and FR901469 productivity. Methodology: We performed genome sequence analysis of mutant strains and detected mutated genes. Subsequently, we classified mutated genes into functional categories and searched the categories in which mutated genes were accumulated as generations progressed. Results: We found that genome regions of two scaffolds were amplified and one of those contained putative FR901469 biosynthesis gene cluster. Moreover, we detected totally 396 mutated genes from 14 mutant strains and the genes within the “Replication, recombination and repair”, “Signal transduction mechanisms” and “Transcription” categories accumulated this mutation. Conclusion: Our study suggests that productivity improvement occurs via the following two mechanisms: the amplification of putative FR901469 biosynthesis gene cluster and mutations of genes categorized as “Replication, recombination and repair”, “Signal transduction mechanisms” and “Transcription”.en_US
dc.identifier.citationMatsui Makoto, Yokoyama Tatsuya, Kumagai Toshitaka, Nemoto Kaoru, Terai Goro, Machida Masayuki, Shibata Takashi, Aburatani Sachiyo. Genomic Analysis of Fungal Species No.11243 Mutant Strains Provides Insights into the Relationship between Mutations and High Productivity. British Biotechnology Journal. 2016; 10(2): 1-13.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/180030
dc.language.isoenen_US
dc.source.urihttps://www.sciencedomain.org/metrics/11692en_US
dc.subjectFR901469en_US
dc.subjectfungal sp. No.11243en_US
dc.subjectgenomic analysisen_US
dc.subjectmutationen_US
dc.titleGenomic Analysis of Fungal Species No.11243 Mutant Strains Provides Insights into the Relationship between Mutations and High Productivity.en_US
dc.typeArticleen_US
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