In vitro clonal multiplication of an apple rootstock by culture of shoot apices and axillary buds.

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dc.contributor.authorKaushal, Nen_US
dc.contributor.authorModgil, Men_US
dc.contributor.authorThakur, Men_US
dc.contributor.authorSharma, D Ren_US
dc.date.accessioned2009-05-28T10:59:56Z
dc.date.available2009-05-28T10:59:56Z
dc.date.issued2005-06-05en_US
dc.description.abstractIn vitro clonal multiplication of apple rootstock MM 111 using axillary buds and shoot apices were carried out. Vegetative axillary buds of the size of 0.2-2.0 cm and shoot apices measuring 4 mm in length were initiated to shoot proliferation on MS medium supplemented with BA (0.5 - 1.0 mgl(-1)), GA3(0.5 mgl(-1)), with or without IBA(0.05 - 0.1 mgl(-1)). Small size explants showed less phenol exudation and less contamination. Following establishment phase, the small shoots emerged from explants were subcultured on MS medium supplemented with different combinations and concentrations of growth regulators. BA (1.0 mgl(-1)) and GA3 (0.5 mgl(-1)) combination showed highest multiplication rate (1:5), andcl also produced longer shoots. Two step rooting was done by transferring microcuttings to auxin free solid medium after root initiation in dark on 1/2 strength MS liquid medium containing IBA (0.5 mgl(-1) ). Rooted plantlets were transferred to peat containing paper cups and resulting plants of MM 111 acclimated successfully for transfer to field.en_US
dc.description.affiliationDepartment of Biotechnology, Dr YS Parmar University of Horticulture & Forestry. Nauni, Solan 173 230, India.en_US
dc.identifier.citationKaushal N, Modgil M, Thakur M, Sharma DR. In vitro clonal multiplication of an apple rootstock by culture of shoot apices and axillary buds. Indian Journal of Experimental Biology. 2005 Jun; 43(6): 561-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/56095
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshCell Culture Techniques --methodsen_US
dc.subject.meshCloning, Molecular --methodsen_US
dc.subject.meshCulture Media --pharmacologyen_US
dc.subject.meshCulture Techniquesen_US
dc.subject.meshFruiten_US
dc.subject.meshIndoleacetic Acidsen_US
dc.subject.meshMalus --cytologyen_US
dc.subject.meshPlant Growth Regulators --metabolismen_US
dc.subject.meshPlant Leaves --metabolismen_US
dc.subject.meshPlant Roots --metabolismen_US
dc.subject.meshPlant Shoots --metabolismen_US
dc.titleIn vitro clonal multiplication of an apple rootstock by culture of shoot apices and axillary buds.en_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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