Purification of heparin binding oviduct specific proteins and its effect on in vitro embryo development in cattle.

Simple item page
dc.contributor.authorSharma, Aditya K
dc.contributor.authorMohapatra, Sushil K
dc.contributor.authorMohanty, A K
dc.contributor.authorDas, S K
dc.date.accessioned2013-07-31T04:53:11Z
dc.date.available2013-07-31T04:53:11Z
dc.date.issued2013-05
dc.description.abstractThe objective of this study was to see the effect of purified heparin binding oviduct specific proteins (OSP) as media supplement on in vitro embryo developmental competence in cattle. The oviduct specific proteins were isolated from abattoir cattle oviducts and precipitated, dialyzed and at the end purified by high performance liquid chromatography system. The SDS-PAGE profile of eluted heparin binding protein (HBP) fraction showed bands between ~66 - ~97 kDa, while heparin unbinding protein (HUBP) fraction showed two bands at ~66 kDa and in total protein (TP) bands were ~60 - ~95 kDa. Collected all three OSP fractions were used as a media supplement in three different concentrations (0, 5 and 20 µg/mL) for in vitro maturation of immature oocytes, in vitro fertilization and culture of presumptive embryos at 38.5 ºC in 5% CO2 incubator with maximum humidity. The highest cleavage rate (73.40±2.36%) was observed at 5 µg/mL concentration level and lowest cleavage rate (27.63±1.89%) was obtained in 20 μg/mL total protein (TP) fraction. The highest blastocyst formation (26.47±1.47%) also occurred in 5 µg/mL concentration of total protein (TP) fraction and the lowest blastocyst rate (3.60±1.80%) was achieved at 20 µg/mL HBP fraction. The highest cleavage rate in the control group was 60.45±2.66% in TP fraction and blastocyst formation was 11.66±2.54% in HUBP fraction which was not significantly differ from HBP fraction. These results indicate that at 5 µg/mL of total OSP fraction (TP) and HBP used as media supplement increased the cleavage rate significantly as compared to HUBP fraction, and total OSP fraction (TP) increased blastocyst formation significantly (P<0.05) as compared to HBP & HUBP fraction.en_US
dc.identifier.citationSharma Aditya K, Mohapatra Sushil K, Mohanty A K, Das S K. Purification of heparin binding oviduct specific proteins and its effect on in vitro embryo development in cattle. Indian Journal of Experimental Biology. 2013 May; 51(5): 347-351.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/147600
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/17673en_US
dc.subjectCattleen_US
dc.subjectEmbryoen_US
dc.subjectIn vitro fertilizationen_US
dc.subjectOviduct specific proteinsen_US
dc.subject.meshAnimals
dc.subject.meshCattle
dc.subject.meshChromatography, High Pressure Liquid
dc.subject.meshElectrophoresis, Polyacrylamide Gel
dc.subject.meshEmbryonic Development
dc.subject.meshFemale
dc.subject.meshHeparin --metabolism
dc.subject.meshIn Vitro Techniques
dc.subject.meshMale
dc.subject.meshOviducts --metabolism
dc.subject.meshProteins --metabolism
dc.subject.meshSperm-Ovum Interactions
dc.titlePurification of heparin binding oviduct specific proteins and its effect on in vitro embryo development in cattle.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijeb2013v51n5p347.pdf
Size:
111.57 KB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Indian Journal of Experimental Biology