Transfer of toxin - coding megaplasmid of bacillus thuringiensis subsp. thompsoni into bacillus sphaericus.

Simple item page
dc.contributor.authorSurendran, A
dc.contributor.authorVennison, S John
dc.date.accessioned2015-08-20T07:30:26Z
dc.date.available2015-08-20T07:30:26Z
dc.date.issued2011-07
dc.description.abstractMosquito borne diseases form a major component of vector borne diseases from all over the world. Several control strategies have been adopted to control diseases transmitted by mosquitoes. Bacterial insecticides have been used for the control of nuisance and vector mosquitoes for more than two decades. Nevertheless, due primarily to their high cost and often only moderate efficacy, these insecticides remain of limited use in tropical countries where mosquito-borne diseases are prevalent. Recently, however, recombinant DNA techniques have been used to improve bacterial insecticide efficacy by markedly increasing the synthesis of mosquitocidal proteins and by enabling new endotoxin combinations from different bacteria to be produced within single strains. Both Bacillus sphaericus and Bacillus thuringiensis subsp.thompsoni produce insecticidal toxins during sporulation and are extensively used in the field for control of mosquito populations. All the known toxins of the latter organism are known to be encoded on its megaplasmids. In an attempt to combine the best properties of the two bacteria, a cry proteins (namely 34- and 40-kDa) encoding megaplasmid (~ 100 MDa) of Bt subsp.thompsoni was transferred to Bacillus sphaericus by conjugation. Many of the transconjugants reacted with antibody to the 34- and 40-kDa Bt subsp.thompsoni crystal toxins in western blotting and were more toxic to Aedes mosquitoes than the wild type B. sphaericus. The toxicity of the transconjugants was maintained through many transfers in the absence of selective pressure.en_US
dc.identifier.citationSurendran A, Vennison S John. Transfer of toxin - coding megaplasmid of bacillus thuringiensis subsp. thompsoni into bacillus sphaericus. International Journal of Applied Biology and Pharmaceutical Technology. 2011 Jul-Sept; 2(3): 366-373.en_US
dc.identifier.issn0976-4550
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/162161
dc.language.isoenen_US
dc.source.urihttps://www.ijabpt.com/Details.aspx?fid=391en_US
dc.subjectBacillus.sphaericusen_US
dc.subjectBt subsp.thompsonien_US
dc.subjecttransconjugationen_US
dc.subjectinsecticidal toxinsen_US
dc.subjectmegaplamiden_US
dc.titleTransfer of toxin - coding megaplasmid of bacillus thuringiensis subsp. thompsoni into bacillus sphaericus.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijabpt2011v2n3p366.pdf
Size:
305.78 KB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
International Journal of Applied Biology and Pharmaceutical Technology