Isolation, Identification and Antibacterial Activity of Endophytic Bacteria from Leaves of Acacia catechu
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Date
2020-06
Journal Title
Journal ISSN
Volume Title
Publisher
Association of Mastitis
Abstract
Drug resistance in bacteria is becoming an alarming problem in recent years. Post-antibiotic era is not so far. The need for novel antibiotics, chemotherapeutic agents and agrochemicals are utmost. Endophytes are microorganisms that reside in the tissues of living plants which are relatively unstudied and are potential sources of novel natural products for exploitation in medicine, agriculture and industry. The endophytes of medicinal plants participate in biochemical pathways and produce novel bioactive compounds. The aim of the study was to isolate, identify and to assess antibacterial activity of endophytic bacteria from leaves of Acacia catechu. 18 endophytic bacteria had been isolated and identified by sequencing 16S rRNA gene. The isolated endophytic bacteria were identified as Bacillus humi. Considerable in vitro antibacterial activities against a panel of human pathogenic microorganisms (Staphylococcus aureus, Streptococcus pyogenes, Bacillus cereus, Klebsiella pneumoniae, Salmonella Typhimurium and Escherichia coli) were recorded. Among 18 isolates, 15 isolates had shown inhibitory activity against Staphylococcus aureus and Salmonella Typhimurium and 10 isolates had shown inhibitory activity against Klebsiella pneumoniae
Description
Keywords
Acacia catechu, endophytic bacteria, 16S rRNA gene, Bacillus humi
Citation
Soman Swathi, Sharma R.K., Sharma Varsha, Gautam Vidhi, Shrman K., Sawarkar Alka, Kewat Rituraj, Kumar Manish. Isolation, Identification and Antibacterial Activity of Endophytic Bacteria from Leaves of Acacia catechu. Journal of Animal Research. 2019 Jun; 10(3): 397-403