(1-3)(1-6)-D-glucans Modulate Immune Status and Blood Glucose Levels in Dogs.
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Date
2014-04
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Abstract
Aims: The objective of this study is to evaluate the effects of adding two different glucans
coded as BGO1 and BGO2 into commercial feed of dogs.
Study Design: We measured changes in phagocytosis, levels of IL-2 in blood, antibody
formation and level of blood sugar (normal homeostasis and experimentally-induced
hyperglycaemie with streptozotocin).
Place and Duration of Study: Department of Pathology, University of Louisville, and
Department of Research and Development, Biorigin, June 2012 and May 2013.
Methodology: The technique employing phagocytosis of synthetic polymeric
microspheres was used for evaluation of phagocytic activity. IL-2 production was
evaluated in serum by an ELISA kit. Formation of antibodies was tested using ovalbumin
as an antigen, level of specific antibodies was measured by ELISA. Blood sugar
evaluation was done both in normal animals and in animals with experimentally-induced
hyperglycaemie. The level of glucose in serum was measured by Antech Diagnostics.
Results: In phagocytosis, both glucans significantly (P ≤0.05 level) increased the
phagocytic activity of blood monocytes and neutrophils. Both glucans were active in IL-2
tests, but BG01 activity was 160% of that of BG02. Similar data were achieved in
evaluation of effects on antibody response – BG01 reached OD of 0.717, whereas BG02
reached OD 0.411. Blood sugar evaluations showed no effect of glucan on normal dogs,
but significant (P ≤0.05 level) reduction in dogs with hyperglycaemie (both glucans
showed same activity). Conclusion: Our data showed that both glucans had significant immunomodulating
effects in immune reactions in the dog model, strongly suggesting that supplementation of
feed with these glucans results in improvement of biological and immunological conditions
of dogs.
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Keywords
Dogs, glucan, phagocytosis, immunity, blood sugar, IL-2, diabetes
Citation
Vetvicka Vaclav, Oliveira Carlos. (1-3)(1-6)-D-glucans Modulate Immune Status and Blood Glucose Levels in Dogs. British Journal of Pharmaceutical Research. 2014 Apr; 4(8): 981-991.