14-3-3 proteins mediate the localization of Centrin2 to centrosome
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Date
2019-06
Authors
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Journal ISSN
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Publisher
Indian Academy of Sciences
Abstract
14-3-3e and 14-3-3c localize to the centrosome and regulate centrosome duplication, by inhibiting cdc25C function. As14-3-3c and 14-3-3e form a complex with centrosomal proteins, we asked if this ability was required to regulate centrosomeduplication. The results in this report demonstrate that 14-3-3e and 14-3-3c form a complex with Centrin2 and that thebinding site is located in the N-terminal EF hand in Centrin2, EF1. A Centrin2 mutant that does not form a complex with14-3-3 proteins displays a punctate cytoplasmic localization and does not localize to the centrosome. These results suggestthat in addition to negatively regulating centrosome duplication as previously reported, 14-3-3 proteins might also berequired for centriole biogenesis by regulating the localization of Centrin2 at the centrosome.
Description
Keywords
Centrosome, centriole, Centrin2, 14-3-3
Citation
Boseh Arunabha, Dalal Sorab N. 14-3-3 proteins mediate the localization of Centrin2 to centrosome. Journal of Biosciences. 2019 Jun; 44(2): 1-10