Detection of an ascorbate radical in an irradiated mice using electron spin resonance (ESR).

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dc.contributor.authorIsoda, Hen_US
dc.contributor.authorAkagi, Ken_US
dc.contributor.authorAoki, Yen_US
dc.contributor.authorTanaka, Yen_US
dc.date.accessioned2009-05-28T12:10:45Z
dc.date.available2009-05-28T12:10:45Z
dc.date.issued1996-09-01en_US
dc.description.abstractAscorbate radical (Asc.-) produced by the reaction of AscH- (ascorbic acid) with HO or O2.- after irradiation in mice has been measured. It is possible to measure Asc.- easily using ESR and a dialysis method in which Asc.- is collected at room temperature in the interstitial fluid through the dialysis membrane. After irradiation, Asc.- increases in both normal muscle and tumor tissues (SCC-VII) in proportion to the radiation dose. These results suggest that the amount of HO and O2.- produced is reflected in the Asc.- production. This method has been found more useful for the following reasons, (i) no special treatment, such as freezing of the sample, and no administration of noxious agents are necessary to measure Asc.-, (ii) irradiation using a dose of only a few Gy shows an increase in production of Asc.-, and (iii) this method dose not require removal of organs. Using this method, Asc.- can serve as an indicator of the amount of HO and O2.- produced by irradiation in vivo.en_US
dc.description.affiliationDepartment of Radiology, Kansai Medical University, Osaka, Japan.en_US
dc.identifier.citationIsoda H, Akagi K, Aoki Y, Tanaka Y. Detection of an ascorbate radical in an irradiated mice using electron spin resonance (ESR). Indian Journal of Experimental Biology. 1996 Sep; 34(9): 898-900en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/57730
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAscorbic Acid --analysisen_US
dc.subject.meshElectron Spin Resonance Spectroscopyen_US
dc.subject.meshFree Radicalsen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C3Hen_US
dc.subject.meshNeoplasm Transplantationen_US
dc.subject.meshRadiation Dosageen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleDetection of an ascorbate radical in an irradiated mice using electron spin resonance (ESR).en_US
dc.typeJournal Articleen_US
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